Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Antibody > Monoclonal Antibodies > Rabbit Anti-Human Dickkopf 1 Homolog Monoclonal Antibody

Rabbit Anti-Human Dickkopf 1 Homolog Monoclonal Antibody

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"DKK1" Related Products.
Cat.No.:
CAB11524RH
Product Overview:
Rabbit Monoclonal Antibody to Human Dickkopf 1 Homolog
Antigen Description:
DKK1 ( dickkopf related protein 1 ) is a head inducer secreted from the vertebrate head organizer and belongs to the DKK protein family including four members. DKK1 is synthesized initially as a precursor protein with an N-terminal signal peptide and two cysteine-rich domains. DKK proteins have distinct patterns of expression in adult and embryonic tissues and have effects on Wnt signaling implicated in diverse processes. Dkk1 is a specific and high-affinity ligand for LRP6 ( LDL-receptor-related protein 6 ) acting as a Wnt co-receptor. Dkk1 blocks LRP6-mediated Wnt/beta-catenin signaling by antagonistically inhibiting the formation of Wnt-Fz-LRP6 complex which triggers the Wnt signal pathway. It is revealed that Wnt signaling in osteoblasts is necessary to promote BMP-2-mediated differentiation of pre-osteoblast cells, and therefore, DKK1 may play a key role in regulating pre-osteoblast differentiation and the development of multiple myeloma-associated osteolytic bone lesions.
Specificity:
Human Dkk1 / Dickkopf-1. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen:
Recombinant human Dkk1 protein
Host:
Rabbit
Isotype:
Rabbit IgG
Species:
Human
Clone:
A005
Applications:
WB; ELISA
Dilution:
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human Dkk1 in WB. Using a DAB detection system, the detection limit for Dkk1 is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommendedELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Dkk1. The detection limit for human Dkk1 is approximately 0.00975 ng/well
Preparation:
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human dickkopf related protein 1.
Format:
0.2 μm filtered solution in PBS with 5% trehalose
Storage:
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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