| Antigen Description : |
Mouse coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor ( TF ). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. The use of warfarin or similar anticoagulants impairs its function. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. The most important substrates for FVIIa-TF are Factor X and Factor IX. |
| Specificity : |
Mouse FVII / Factor VII / F7. No cross-reactivity in WB and ELISA with Human F10 / FX / Factor X, Human F11 / FXI / Factor XI, Human Kallikrein-1 / KLK-1, Human Kallikrein-13 / KLK-13, Human Kallikrein-11 / KLK-11, Human Kallikrein-7 / KLK-7, Human cell lysate (293 cell line) |
| Immunogen : |
Recombinant mouse FVII protein |
| Host : |
Rabbit |
| Isotype : |
Rabbit IgG |
| Species : |
Mouse |
| Clone : |
A122 |
| Applications : |
WB; ELISA |
| Dilution : |
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse FVII in WB. Using a DAB detection system, the detection limit for mouse FVII is approximately 0.125 ng/lane under non-reducing conditions and reducing conditionsELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse FVII. The detection limit for mouse FVII is approximately 0.00245 ng/well |
| Preparation : |
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse FVII |
| Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
| Storage : |
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
