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Rabbit Monoclonal Antibody to Human Nicastrin

Cat.No.: CAB11585RH
Product Overview: Rabbit Monoclonal Antibody to Human Nicastrin
Antigen Description: Nicastrin, also known as NCSTN, is a single-pass type I membrane protein which belongs to the nicastrin family. Nicastrin was named after the Italian village Nicastro, reflecting the fact that Alzheimers disease was described in 1963 after studying descendants of an extended family originating in the village of Nicastro that had Familial Alzheimers Disease (FAD). Nicastrin is a protein that is part of the gamma secretase protein complex, which is one of the proteases involved in processing amyloid precursor protein (APP) to the short Alzheimers disease-associated peptide amyloid beta. Nicastrin may represent a stabilizing cofactor required for the assembly of the gamma-secretase complex. The other proteins in the complex are presenilin, which is the catalytically active component of the complex, APH-1, and PEN-2. Nicastrin itself is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the complex, all of which undergo significant post-translational modification before becoming active in the cell. Nicastrin has also been identified as a regulator of neprilysin, an enzyme involved in the degradation of amyloid beta fragment.
Specificity: Human Nicastrin / NCSTN. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen: Recombinant Human Nicastrin / NCSTN Protein
Host: Rabbit
Isotype: Rabbit IgG
Species: Human
Clone: A008
Applications: Western blot; ELISA
Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NCSTN in WB. Using a DAB detection system, the detection limit for Human NCSTN is approximately 10 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human NCSTN. The detection limit for Human NCSTN is approximately 0.0049 ng/well.
Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human Nicastrin / NCSTN
Format: 0.2 μm filtered solution in PBS with 5% trehalose
Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name: NCSTN nicastrin [ Homo sapiens ]
Official Symbol: NCSTN
Synonyms: NCSTN; nicastrin; APH2; KIAA0253; ATAG1874; anterior pharynx-defective 2; RP11-517F10.1;
Gene ID: 23385
mRNA Refseq: NM_015331
Protein Refseq: NP_056146
MIM: 605254
UniProt ID: Q92542
Chromosome Location: 1q22-q23
Pathway: Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; NRIF signals cell death from the nucleus, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem;
Function: aspartic-type endopeptidase activity; endopeptidase activity; peptidase activity; protein binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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