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Mouse Monoclonal Antibody to Human Tyrosine Kinase With Immunoglobulin-like And EGF-like Domains 1

Cat.No. : CAB11615MH
Product Overview : Mouse Monoclonal Antibody to Human Tyrosine Kinase With Immunoglobulin-like And EGF-like Domains 1
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  • Gene Information
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Antigen Description : Tyrosine-protein kinase receptor Tie-1, also known as TIE1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, Tyr protein kinase family and Tie subfamily. TIE1 contains three EGF-like domains, three fibronectin type-III domains, two Ig-like C2-type ( immunoglobulin-like ) domains and one protein kinase domain. TIE1 is an endothelial-cell-specific tyrosine kinase receptor. It is a cell surface protein expressed exclusively in endothelial cells. It is required for the survival and growth of microvascular endothelial cells during the capillary sprouting phase of vascular development. TIE1 is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. TIE1 upregulates the cell adhesion molecules (CAMs) VCAM-1, E-selectin, and ICAM-1 through a p38-dependent mechanism. Attachment of monocyte derived immune cells to endothelial cells is also enhanced by TIE1 expression. TIE1 has a proinflammatory effect and may play a role in the endothelial inflammatory diseases such as atherosclerosis.
Specificity : Human Tie1. No cross-reactivity in ELISA with Human Tie2; Human cell lysate (293 cell line)
Immunogen : Recombinant Human Tie1 Protein
Host : Mouse
Isotype : Mouse IgG2b
Species : Human
Clone : 9E3E3
Applications : Western blot
Dilution : This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human TIE1 in WB. Using a DAB detection system, the detection limit for Human TIE1 is approximately 8 ng/lane under non-reducing conditions and reducing conditions.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human Tie1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : TIE1 tyrosine kinase with immunoglobulin-like and EGF-like domains 1 [ Homo sapiens ]
Official Symbol : TIE1
Synonyms : TIE1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; TIE,tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; tyrosine-protein kinase receptor Tie-1; JTK14; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; TIE;
Gene ID : 7075
mRNA Refseq : NM_001253357
Protein Refseq : NP_001240286
MIM : 600222
UniProt ID : P35590
Chromosome Location : 1p34-p33
Function : ATP binding; nucleotide binding; protein binding; protein tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How can I increase the expression level of TIE1 protein? 02/25/2022

At present, there is no direct way to increase the expression level of TIE1 protein, but it can be improved by regulating gene expression and providing nutritional support.

What are the applications of TIE1 protein in drug discovery? 12/10/2020

This protein can be used as a target for the development of new drugs, or as a drug carrier for the targeted delivery of drugs.

What is the current state of development for TIE1? 12/04/2020

TIE1 is currently in clinical trials, including small molecule inhibitors against TIE1 and inhibitors against the TIE1 signaling pathway.

How can TIE1 be used as a biomarker in clinical practice? 06/07/2020

In clinical practice, the expression level of TIE1 can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of angiogenesis-related diseases, measuring the expression level of TIE1 can help doctors determine the type, stage, and prognosis of the disease, and guide the choice of treatment regimen.

What is the significance of studying the TIE1 protein? 05/06/2020

TIE1 protein can shed light on the mechanisms of angiogenesis and embryonic development, as well as the pathogenesis of certain angiogenesis-related diseases. In addition, it is of great significance for the development of new drugs and treatment strategies.

How about TIE1 protein related to autoimmune diseases? 02/21/2020

Studies have shown that TIE1 protein plays an important role in the pathogenesis of certain autoimmune diseases.

Customer Reviews (3)

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12/19/2022

    After repeated use, it can also maintain the stability of the performance, which makes me more confident in the experimental results.

    06/11/2022

      Green and pollution-free, it can be seen that the manufacturer is very environmentally conscious.

      01/29/2021

        The range of applications is breathtaking, and it can play an important role in any field.

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