Recombinant Fire fly luciferase produced in E. coli has a molecular mass of approximately 62 KDa.
Luciferases are enzymes that emit light. A diverse group of organisms use luciferase-mediated bioluminescence to startle predators or to attract prey or mates. The luciferase from the North American fire fly releases green light during the oxidation of its chemical substrate, luciferin. Recombinant luciferase is expressed inE. coli.
>95% as determined by SDS-PAGE.
more than 2.0×1011RLU/mg.
This lyophilized preparation should be kept at –20°C for six months, and kept at –70°C for one year.
Crystal structure ofPhotinus pyralisfirefly luciferase.