Recombinant Fire fly luciferase produced in E. coli has a molecular mass of approximately 62 KDa.
Luciferases are enzymes that emit light. A diverse group of organisms use luciferase-mediated bioluminescence to startle predators or to attract prey or mates. The luciferase from the North American fire fly releases green light during the oxidation of its chemical substrate, luciferin. Recombinant luciferase is expressed in E. coli.
>95% as determined by SDS-PAGE.
more than 2.0×1011 RLU/mg.
>95% as determined by SDS-PAGE..
This lyophilized preparation should be kept at –20°C for six months, and kept at –70°C for one year.
Crystal structure of Photinus pyralis firefly luciferase.