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Home > Products > Recombinant Proteins > Recombinant Hirudin

Product Description

Cat. No.

Hirudin-02

Product Overview

Recombinant Hirudin produced in Pichia. Pastoris contains 65 amino acid residues and having a molecular mass of 6979.5 Da.

Description

Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63.

Purity

>96% by SDS-PAGE and HPLC analyses.

CAS Number

8001-27-2

Formulation

Lyophilized from a 0.2μm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Production

Yeast expression of a DNA sequence encoding the mature hirudin variant (Leu1,Thr2-63-desulfo hirudin).

Specific Activity

rHirudin is fully biologically active when compared to standard. Its specific activity is ≥1×104ATU/mg.

Endotoxin

Less than 10EU/mg of rhirudin as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable at 2-8oC, but should be kept at -20oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC. Avoid repeated freeze/thaw cycles.

 




 

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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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