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"Hirudin" Related Products.
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Home > Products > Recombinant Proteins > Recombinant Hirudin

Recombinant Hirudin

Cat. No.:
Hirudin-02
Product Overview:
Recombinant Hirudin produced inPichia. Pastoriscontains 65 amino acid residues and having a molecular mass of 6979.5 Da.
Description:
Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63.
Purity:
>96% by SDS-PAGE and HPLC analyses.
CAS Number:
8001-27-2
Formulation:
Lyophilized from a 0.2μm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Production:
Yeast expression of a DNA sequence encoding the mature hirudin variant (Leu1,Thr2-63-desulfo hirudin).
Specific Activity:
rHirudin is fully biologically active when compared to standard. Its specific activity is ≥1×104ATU/mg.
Endotoxin:
Less than 10EU/mg of rhirudin as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8oC, but should be kept at -20oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC. Avoid repeated freeze/thaw cycles.
 
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Ordering Information Distributors
1-631-559-9269
1-631-448-7888
1-631-938-8127
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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