Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 18.104.22.168) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
Sterile white lyophilized powder. The protein (0.5 mg/ml) was dialyzed against 5 mM sodium citrate, 0.01% PEG, and 0.02 M NaCl pH 6.5 before lyophilization.
The specific activity was found to be 2000IU/mg.
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
HuThrombin although stable at room temperature for 4°C, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
F2 coagulation factor II (thrombin) [ Homo sapiens ]
F2; coagulation factor II (thrombin); prothrombin; serine protease; prothrombin B-chain; PT; THPH1;
Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Blood Clotting Cascade, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Common Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem;
calcium ion binding; growth factor activity; peptidase activity; protein binding; receptor binding; contributes_to receptor binding; serine-type endopeptidase activity; thrombospondin receptor activity;