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Home > Products > Recombinant Proteins > Human Thrombin

Human Thrombin

Cat.No.:
Thrombin-22H
Product Name:
Human Thrombin
Description:
Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.
Species:
Human
Form:
Sterile white lyophilized powder. The protein (0.5 mg/ml) was dialyzed against 5 mM sodium citrate, 0.01% PEG, and 0.02 M NaCl pH 6.5 before lyophilization.
Bio-activity:
The specific activity was found to be 2000IU/mg.
Purity:
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Storage:
HuThrombin although stable at room temperature for 4°C, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.


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1-631-559-9269
1-631-448-7888
1-631-938-8127
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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