Recombinant Human PLAT protein(Tyr37-Arg310), hFc-tagged

• Cat.No.: PLAT-8525H
• Product Overview: Recombinant Human tPA (Tyr 37-Arg 310)(NP_000921.1) was expressed in HEK293 with the fused Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Tyr37-Arg310
• Form: Lyophilized from sterile 50mM NaAc, 150mM NaCl, 5mM CaCl2, 10% glycerol, PH 5.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human Fc/tPAα is a disulfide-linked homodimeric protein. The reduced monomer consists of 512 amino acids and has a predicted molecular mass of 57.5 KDa. As a result of glycosylation, the apparent molecular mass of rh Fc/tPAα monomer is approximately 59 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PLAT plasminogen activator, tissue [ Homo sapiens ]
• Official Symbol: PLAT
• Synonyms: PLAT; plasminogen activator, tissue; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); TPA; T-PA; DKFZp686I03148
• Gene ID: 5327
• mRNA Refseq: NM_000930
• Protein Refseq: NP_000921
• MIM: 173370
• UniProt ID: P00750