Active Recombinant Human LGMN, His-tagged

• Cat.No.: LGMN-7980H
• Product Overview: Recombinant Human LGMN (Ile18-Tyr433) fused with an N-terminal 7-His tag, was expressed in Mouse NSO Cells.

Specification

• Description: This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >250 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass:49 kDa SDS-PAGE:60 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 3 months from date of receipt, -70 °C as supplied. 1 month, -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: LGMN legumain [ Homo sapiens (human) ]
• Official Symbol: LGMN
• Synonyms: LGMN; AEP; LGMN1; PRSC1; legumain; cysteine protease 1; protease, cysteine 1; asparaginyl endopeptidase; protease, cysteine, 1 (legumain); EC 3.4.22.34
• Gene ID: 5641
• mRNA Refseq: NM_005606
• Protein Refseq: NP_005597
• MIM: 602620
• UniProt ID: Q99538
• Chromosome Location: 14q32.1
• Pathway: Adaptive Immune System; Antigen processing and presentation; Immune System
• Function: cysteine-type endopeptidase activity; peptidase activity