Recombinant Human PDGFAA

• Cat.No.: PDGFA-56H
• Product Overview: Recombinant Human PDGF-AA produced inYeastis a homodimeric, glycosilated, polypeptide chain containing 2 x 110 amino acids and having a total molecular mass of 34 kDa. PDGF-AA is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PDGFA-56H
• Description: PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
• Source: Yeast.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by SDS-PAGE.
• Formulation: The protein was lyophilized with 20mM sodium phosphate buffer.
• Solubility: It is recommended to reconstitute the lyophilized PDGF-AA in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀was found to be 1ng/ml calculated by the ability to stimulate the proliferation of mouse 3T3 fibroblasts. (PNAS 94, 10205, 1997. Biochemistry, 1996, 35, 12077).
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.
• Storage: Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PDGFA platelet-derived growth factor alpha polypeptide [ Homo sapiens ]
• Synonyms: PDGFA; platelet-derived growth factor alpha polypeptide, 2 x 110 amino acids; PDGF1; PDGF-A; Platelet-derived growth factor subunit A; PDGF subunit A; Platelet-derived growth factor A chain; PDGF A-chain; PDGF subunit A; platelet-derived growth factor alpha; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform; preproprotein
• Gene ID: 5154
• mRNA Refseq: NM_002607
• Protein Refseq: NP_002598
• UniProt ID: P04085
• Chromosome Location: 7p22
• MIM: 173430
• Pathway: Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Prostate cancer; Regulation of actin cytoskeleton; Hemostasis; Signaling by PDGF
• Function: cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; contributes_to platelet-derived growth factor receptor binding; protein heterodimerization activity