Recombinant Human queuine tRNA-ribosyltransferase domain containing 1, His-tagged

• Cat.No.: QTRTD1-645H
• Product Overview: QTRTD1, 1-415aa Human, His tag, E.coli

Specification

• Description: QTRTD1 interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine. Recombinant human QTRTD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In Phosphate buffer saline (pH 7.4) containing 30% glycerol, 2mM DTT, 1mM EDTA, 0.1mM PMSF
• Molecular Mass: 49.1kDa (438aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKLSLTK VVNGCRLGKI KNLGKTGDHT MDIPGCLLYT KTGSAPHLTH HTLHNIHGVP AMAQLTLSSL AEHHEVLTEY KEGVGKFIGM PESLLYCSLH DPVSPCPAGY VTNKSVSVWS VAGRVEMTVS KFMAIQKALQ PDWFQCLSDG EVSCKEATSI KRVRKSVDRS LLFLDNCLRL QEESEVLQKS VIIGVIEGGD VMEERLRSAR ETAKRPVGGF LLDGFQGNPT TLEARLRLLS SVTAELPEDK PRLISGVSRP DEVLECIERG VDLFESFFPY QVTERGCALT FSFDYQPNPE ETLLQQNGTQ EEIKCMDQIK KIETTGCNQE ITSFEINLKE KKYQEDFNPL VRGCSCYCCK NHTRAYIHHL LVTNELLAGV LLMMHNFEHY FGFFHYIREA LKSDKLAQLK ELIHRQAS
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: QTRTD1 queuine tRNA-ribosyltransferase domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: QTRTD1
• Synonyms: QTRTD1; queuine tRNA-ribosyltransferase domain containing 1; queuine tRNA-ribosyltransferase subunit QTRTD1; queuine tRNA-ribosyltransferase domain-containing protein 1; NP_001243764.1; EC 2.4.2.29; NP_001243765.1; NP_001243766.1; NP_078914.1
• Gene ID: 79691
• mRNA Refseq: NM_001256835
• Protein Refseq: NP_001243764
• UniProt ID: Q9H974
• Chromosome Location: 3q13.31
• Function: contributes_to queuine tRNA-ribosyltransferase activity; zinc ion binding