Recombinant Human TIMP3 protein, Fc-tagged
Recombinant Human TIMP3(Cys24-Pro211) fused with Fc region of human IgG1 at N-terminal was expressed in HEK293.
Lyophilized from 0.2μm-filtered solution in PBS.
Measured by its ability to induce alkaline phosphatase production.
The predicted molecular mass is 48 kDa, and the apparent molecular mass is approximately 55 kDa in SDS-PAGE under reducing conditions.
<0.06 EU/μg as determined by LAL test.
>95%, by SDS-PAGE under reducing conditions.
Stable for at least 1 year after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade. Avoid freeze/thaw cycles.
Reconstitute with sterile water to a concentration of 1mg/ml. Add 1xPBS to the desired protein concentration.