Active Recombinant Human SETD2, FLAG-tagged

• Cat.No.: SETD2-69H
• Product Overview: Recombinant Human SETD2 (1392-2564) (accession number NP_054878.5) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 134 kDa.

Specification

• Description: SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II.
• Source: Sf9 insect cells
• Species: Human
• Tag: FLAG
• Form: 25 mM HEPES, pH 7.5, 150 mM NaCl and 5% glycerol.
• Bio-activity: H3K36 methyltransferase.
• Applications: Enzyme kinetics, inhibitor screening, and selectivity profiling.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: SETD2 SET domain containing 2 [ Homo sapiens ]
• Official Symbol: SETD2
• Synonyms: SETD2; SET domain containing 2; histone-lysine N-methyltransferase SETD2; FLJ23184; HIF 1; HYPB; KIAA1732; KMT3A; huntingtin yeast partner B; lysine N-methyltransferase 3A; huntingtin interacting protein 1; huntingtin-interacting protein B; SET2; HIF-1; HIP-1; HBP231; HSPC069; p231HBP; FLJ16420; FLJ22472; FLJ45883; FLJ46217
• Gene ID: 29072
• mRNA Refseq: NM_014159
• Protein Refseq: NP_054878
• MIM: 612778
• UniProt ID: Q9BYW2
• Chromosome Location: 3p21.31
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; oxidoreductase activity; protein binding; transferase activity; transition metal ion binding