Recombinant Human CR1(Gln42-Asp1971) fused with a C-terminal 10-His tag and a substitution of His1208Arg was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. When rhCD35 is coated at 5 μg/mL, complement component C3b binds with an apparent KD <10 nM.
Predicted Molecular Mass: 213 kDa;SDS-PAGE: 200-220 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 400 μg/mL in PBS.