Active Recombinant Human SDC1 Protein, His-tagged

• Cat.No.: SDC1-523H
• Product Overview: Recombinant Human SDC1 (Accession # NP_002988) Gln18-Glu251, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained: Gln18 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin.When 5 x 10^4 cells/well are added to human Syndecan-1 and human Fibronectin (0.5 µg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 2.5 - 10 µg/mL.
• Molecular Mass: Recombinant Human SDC1, His-tagged has a calculated MW of 24.9 kDa. In SDS-PAGE migrates as 65-90 kDa, reducing conditions.
• Protein length: Gln18-Glu251
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SDC1 syndecan 1 [ Homo sapiens ]
• Official Symbol: SDC1
• Synonyms: SDC1; syndecan 1; SDC; syndecan-1; CD138; SYND1; syndecan; syndecan proteoglycan 1; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor
• Gene ID: 6382
• mRNA Refseq: NM_001006946
• Protein Refseq: NP_001006947
• MIM: 186355
• UniProt ID: P18827
• Chromosome Location: 2p24.1