Recombinant Human ADAMTS13(Gln34-Thr1427) fused with His tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Brij-35.
Measured by its ability to cleave the fluorogenic peptide substrate, FRETS-VWF73. The specific activity is >10 pmol/min/μg.
Predicted Molecular Mass: 147 kDa;
SDS-PAGE: 160-180 kDa, reducing conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.