| Cat. No. : |
FGF21-104H |
| Description : |
FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways. |
| Source : |
Escherichia Coli. |
| Amino Acid Sequence : |
MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS |
| Form : |
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Molecular Weight : |
21.6 kDa (202aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher) |
| Purity : |
> 90% by SDS - PAGE |
| Concentration : |
1 mg/ml (determined by Bradford assay) |
| Endotoxin Level : |
< 1.0 EU per 1 μg of protein (determined by LAL method) |
| Biological Activity : |
The ED50 for this effect is 1.4 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell. |
| Activity Assay : |
1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay Medium: serum free RPMI 1640 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) 5. Serum Free Starvation: 24hr with RPMI1640 6. Incubation Time: 24 hr (after sample treatment) 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution)8. Detection method: BrdU assay |
| Storage : |
Can be stored at +4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70℃. Avoid repeated freezing and thawing cycles. |
