Active Recombinant Human DPP4 protein, His-tagged

• Cat.No.: DPP4-215H
• Product Overview: Recombinant Human DPP4(39 a.a. - 766 a.a.) fused with His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
• Source: E. coli
• Species: Human
• Tag: His
• Form: In 20 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 8.0 (10% glycerol)
• Bio-activity: Approximately > 50 Unit/mg.
• Molecular Mass: 86.4 kDa
• AA Sequence: ADPRKTYTLTDYLKNTYRLKLYSLR WISDHEYLYKQENNILVFNAEYGNS SVFLENSTFDEFGHSINDYSISPDG QFILLEYNYVKQWRHSYTASYDIYD LNKRQLITEERIPNNTQWVTWSPVG HKLAYVWNNDIYVKIEPNLPSYRIT WTGKEDIIYNGITDWVYEEEVFSAY SALWWSPNGTFLAYAQFNDTEVPLI EYSFYSDESLQYPKTVRVPYPKAGA VNPTVKFFVVNTDSLSSVTNATSIQ ITAPASMLIGDHYLCDVTWATQERI SLQWLRRIQNYSVMDICDYDESSGR WNCLVARQHIEMSTTGWVGRFRPSE PHFTLDGNSFYKIISNEEGYRHICY FQIDKKDCTFITKGTWEVIGIEALT SDYLYYISNEYKGMPGGRNLYKIQL SDYTKVTCLSCELNPERCQYYSVSF SKEAKYYQLRCSGPGLPLYTLHSSV NDKGLRVLEDNSALDKMLQNVQMPS KKLDFIILNETKFWYQMILPPHFDK SKKYPLLLDVYAGPCSQKADTVFRL NWATYLASTENIIVASFDGRGSGYQ GDKIMHAINRRLGTFEVEDQIEAAR QFSKMGFVDNKRIAIWGWSYGGYVT SMVLGSGSGVFKCGIAVAPVSRWEY YDSVYTERYMGLPTPEDNLDHYRNS TVMSRAENFKQVEYLLIHGTADDNV HFQQSAQISKALVDVGVDFQAMWYT DEDHGIASSTAHQHIYTHMSHFIKQ CFSLP-SGRLVPRGSHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Unit Definition: One unit will hydrolyze 1 umole of p-nitroaniline per minute at pH 8.0 at 37 centigrade using 1mM of Gly-Pro p-nitroanilde as a substrate.
• Applications: Functional Study; SDS-PAGE
• Storage: Store at 4 centigrade for 1-2 weeks. For long term storage store at -20 centigrade or -80 centigrade.Aliquot to avoid repeated freezing and thawing.
• Concentration: 0.5 mg/mL

Gene Information

• Gene Name: DPP4 dipeptidyl-peptidase 4 [ Homo sapiens ]
• Official Symbol: DPP4
• Synonyms: DPP4; dipeptidyl-peptidase 4; ADCP2, adenosine deaminase complexing protein 2 , CD26, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); dipeptidyl peptidase 4; DPPIV; ADCP-2; DPP IV; dipeptidylpeptidase 4; dipeptidyl peptidase IV; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); CD26; ADABP; ADCP2; TP103
• Gene ID: 1803
• mRNA Refseq: NM_001935
• Protein Refseq: NP_001926
• MIM: 102720
• UniProt ID: P27487
• Chromosome Location: 2q23-qter
• Pathway: Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Synthesis, Secretion, and Inactivation of Glucagon-like Peptide-1 (GLP-1), organism-specific biosystem
• Function: aminopeptidase activity; collagen binding; dipeptidyl-peptidase activity; peptidase activity; peptide binding; protease binding; protein binding; protein homodimerization activity; receptor activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity