| Cat. No. : |
IFNGR1-22H |
| Description : |
Interferon-gamma (IFN-gamma) is a pleotropic cytokine expressed predominantly by naïve and activated CD8+ and TH1 CD4+ T cells, and natural killer (NK) cells and, as such, promotes both innate and adaptive immune responses.The activity of IFN-gamma is mediated through its receptor, the high-affinity IFN-gamma receptor complex, a class II cytokine receptor that is present on T cells, B cells, macrophages, neutrophils and NK cells as well as non-immune somatic cells such as endothelial cells and fibroblasts. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
EMGTADLGPSSVPTPTNVTIESYNM NPIVYWEYQIMPQVPVFTVEVKNYG VKNSEWIDACINISHHYCNISDHVG DPSNSLWVRVKARVGQKESAYAKSE EFAVCRDGKIGPPKLDIRKEEKQIM IDIFHPSVFVNGDEQEVDYDPETTC YIRVYNVYVRMNGSEIQYKILTQKE DDCDEIQCQLAIPVSSLNSQYCVSA EGVLHVWGVTTEKSKEVCITIFNSS IKGGSSNTKVDKKVEPKSCDKTHTC PPCPAPELLGGPSVFLFPPKPKDTL MISRTPEVTCVVVDVSHEDPEVKFN WYVDGVEVHNAKTKPREEQYNSTYR VVSVLTVLHQDWLNGKEYKCKVSNK ALPAPIEKTISKAKGQPREPQVYTL PPSRDELTKNQVSLTCLVKGFYPSD IAVEWESNGQPENNYKTTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCS VMHEALHNHYTQKSLSLSPGK. |
| Molecular Mass : |
IFN-gamma R1 – Fc Chimera migrates as a broad band between 60 and 85 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. |
| pI : |
IFN-gamma R1 – Fc Chimera separates into a number of glycoforms with a pI between 5 and 8 in 2D PAGE due to post-translational modifications, in particular glycosylation. |
| % Carbohydrate : |
Purified IFN-gamma R1 – Fc Chimera consists of 10-35% carbohydrate by weight. |
| Glycosylation : |
IFN-gamma R1 – Fc Chimera has N- and possibly O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
| Activity : |
The ED50 of IFN-gamma R1 – Fc Chimera is typically 0.05-0.1 μg/ml as measured by its ability to neutralize IFN gamma mediated cytotoxicity using the HT-29 colorectal adenocarcinoma cell line. |
