Recombinant Human OTU Domain, Ubiquitin Aldehyde Binding 1

• Cat.No.: OTUB1-482H
• Product Overview: Recombinant OTUB1 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 33.4 kDa (291aa).

Specification

• Cat. No.: OTUB1-482H
• Description: Otubain 1, also known as OTUB1, is a member of the OUT (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with a ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth.
• Source: E.coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K
• Form: Liquid. In 20 mM Tris buffer (pH 8.0) containing 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: OTUB1 OTU domain, ubiquitin aldehyde binding 1 [ Homo sapiens ]
• Synonyms: OTUB1; OTU domain, ubiquitin aldehyde binding 1; OTB1; OTU1; HSPC263; MGC4584; FLJ20113; FLJ40710; MGC111158;OTU-domain Ubal-binding 1; ubiquitin-specific protease otubain 1; Ubiquitin thioesterase OTUB1; Otubain-1; OTU domain-containing ubiquitin; aldehyde-binding protein 1; Ubiquitin-specific-processing protease; Deubiquitinating enzyme OTUB1
• Gene ID: 55611
• mRNA Refseq: NM_017670
• Protein Refseq: NP_060140
• MIM: 608337
• UniProt ID: Q96FW1
• Chromosome Location: 11q13.1
• Function: cysteine-type peptidase activity; peptidase activity