Recombinant Human Fibroblast Growth Factor 6
Cat.No. : | FGF6-176H |
Product Overview : | This recombinant human FGF-6 is a 18.7 kDa protein containing 168 amino acid residues and this protein expressed inE.Coli. |
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- Gene Information
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Cat. No. : | FGF6-176H |
Description : | Fibroblast Growth Factor-6 (FGF-6) is a newly discovered heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. |
Source : | E.Coli. |
Supplied : | Sterile filtered and the the protein was lyophilized from 10mM Tris pH 7.4 + 100mM NaCl. (no additives). |
Purity : | >95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-6. |
Reconstitution : | The lyophilized FGF-6 should be reconstituted in water to a concentration of 0.5-1mg/ml. Allow to set at least 30 minutes at 4℃, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4°Cfor up to 1 week or stored at -20°Cfor future use. For most in-vitro applications,human FGF-6 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml. |
Note : | If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentaration <10ug/ml. |
Reactivity : | The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <0.5ng/ml, corresponding to a specific activity of >1 X 106 (1 million) units/mg. |
Amino Acid Sequence : | MGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI |
Storage : | The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated below 0°C. Reconstituted human FGF-6 should be stored in working aliquots at -20°C. |
Gene Name : | FGF6 fibroblast growth factor 6 [ Homo sapiens ] |
Synonyms : | FGF6; HBGF-6; HST2; fibroblast growth factor 6; HST-2; Heparin-binding growth factor 6 |
Gene ID : | 2251 |
mRNA Refseq : | NM_020996 |
Protein Refseq : | NP_066276 |
MIM : | 134921 |
UniProt ID : | P10767 |
Chromosome Location : | 12p13 |
Pathway : | MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of actin cytoskeleton |
Function : | growth factor activity |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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