Active Recombinant Human KLK11 protein(Met1-Asn250), His-tagged

• Cat.No.: KLK11-300H
• Product Overview: Recombinant Human KLK11 isoform 1 (NP_006844.1) (Met 1-Asn 250) with a C-terminal polyhistidine tag was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Asn250
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5, 5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al., 1999, J. Biol. Chem. 274: 30468). The specific activity is >200 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Molecular Mass: The secreted recombinant human KLKL11 comprises 243 amino acids with a predicted molecular mass of 27 kDa. As a result of glycosylation, rhKLK11 migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLK11 kallikrein-related peptidase 11 [ Homo sapiens ]
• Official Symbol: KLK11
• Synonyms: KLK11; kallikrein-related peptidase 11; kallikrein 11 , PRSS20; kallikrein-11; TLSP; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease; PRSS20; MGC33060
• Gene ID: 11012
• mRNA Refseq: NM_001136032
• Protein Refseq: NP_001129504
• MIM: 604434
• UniProt ID: Q9UBX7