WD-repeat protein 5

• Cat.No.: CBCRY09

Specification

• Cat. No.: CBCRY09
• Background: The WD40 repeat protein WDR5 specifically associates with the K4-methylated histone H3 in human cells. The structure of WDR5 in complex with a dimethylated H3-K4 peptide was deterimined. Unlike the chromodomain that recognizes the methylated H3-K4 through a hydrophobic cage, the specificity of WDR5 for methylated H3-K4 is conferred by the nonconventional hydrogen bonds between the two zeta-methyl groups of the dimethylated Lys4 and the carboxylate oxygen of Glu322 in WDR5. The three amino acids Ala-Arg-Thr preceding Lys4 form most of the specific contacts with WDR5, with Ala1 forming intermolecular hydrogen bonds and salt bridges, and the side chain of Arg2 inserting into the central channel of WDR5.
• Protein Classification: Structural protein and DNA binding protein
• Structure Weight: 35306.44 Da
• Polymer: 1
• Molecule: WD-repeat protein 5
• Chain Length: 311 amino acids
• PDB ID: 2G9A
• MMDB ID: 41532
• Source: E.coli
• Method: X-Ray Diffraction
• Resolution: 2.7 Å
• Reference: Han Z, Guo L, Wang H, Shen Y, Deng XW, Chai J. Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5. Mol. Cell 2006, 22(1):137-44.

Gene Information

• Gene Name: WDR5
• Synonyms: BIG-3; BIG3; BMP2-induced 3-kb gene protein; SWD3, Set1c WD40 repeat protein, homolog; WD-repeat protein 5; WD repeat domain 5
• UniProt ID: P61964
• Gene ID: 11091
• Chromosome Location: 9q34
• Function: protein binding