Recombinant Human SETD2

• Cat.No.: SETD2-27850TH
• Product Overview: Recombinant fragment, corresponding to amino acids 915-1211of Human KMT3A / HYPB / HIF-1, with N-terminal proprietary tag, 59.7 kDa.

Specification

• Description: Huntingtons disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.
• Source: E. coli
• Biological activity: Specific activity assay conditions: Assay conditions: 80 μlreaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 μM substrate (histone H3), 12.5 μM S-adenosylhomocysteine, and 0-2.5 μM Set2) incubate for 1 hr at 37oC. Add fluorescently labeled tracer a
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: SETD2 SET domain containing 2 [ Homo sapiens ]
• Official Symbol: SETD2
• Synonyms: SETD2; SET domain containing 2; histone-lysine N-methyltransferase SETD2; FLJ23184; HIF 1; HYPB; KIAA1732; KMT3A
• Gene ID: 29072
• mRNA Refseq: NM_014159
• Protein Refseq: NP_054878
• MIM: 612778
• Uniprot ID: Q9BYW2
• Chromosome Location: 3p21.31
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; oxidoreductase activity; transferase activity