Recombinant Human Aggrecan, Interglobular Domain, His-tagged
Cat.No. : | ACAN-2888H |
Product Overview : | The polypeptide connecting human aggrecan globular domains 1 and 2 (T331 - G458 ) is expressed in E. coli with a C-terminal His-tag. The recombinant protein contains cleavage sites for aggrecanases (E373 - A374 in human aggrecan) and matrix metalloproteinases (N341 - F342 in human aggrecan). The calculated Mr of the His-tagged protein is 15 493 Da. |
- Specification
- Gene Information
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Cat. No. : | ACAN-2888H |
Description : | Aggrecan is a large aggregating proteoglycan of articular cartilage. It is also found in aorta, discs and tendons. The aggrecan core protein consists of 2317 amino acids. Up to 130 glucosaminoglycan chains are attached to the core protein and the total molecular mass can reach 2.2 - 3.0 x 106 Daltons. Within the aggrecan molecule 3 globular domains G1, G2 and G3 can be distinguished. Domains G1 and G2 are connected by a rod-shaped polypeptide called interglobular domain (IGD), while the sequence between domains G2 and G3 contains attachment regions for keratan sulfate and chondroitin sulfate chains. Aggrecan interacts via the G1 domain with hyaluronan and link protein to form large aggregates. |
Source : | E.coli. |
Sequences Of Amino Acids : | T A E D F V D I P E N F F G V G G E E D I T V Q T V T W P D M E L P L P R N I T E G E A R G S V I L T V K P I F E V S P S P L E P E E P F T F A P E I G A T A F A E V E N E T G E A T R P W G F P T P G L G P A T A F T S E D L V V Q V T A V P G Q P H L P G G (His-tag) |
Form : | The protein is solubilized in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2. |
Purity : | The recombinant aggrecan interglobular domain appears as a major band at about 21 kDa in SDSPAGE. It represents more than 90 % of total protein in the preparation. |
Applications : | Aggrecan interglobular domain is used as substrate for aggrecanases and matrix metalloproteinases. For proteinase activity measurements, the protein is incubated with proteinase for various time intervals. Thereafter, aliquots of the incubation mixture are analyzed by SDS-PAGE or by ELISA. Upon cleavage with aggrecanases the apparent Mr of aggrecan interglobular domain in SDS-PAGE is reduced from 21 kDa to about 13 kDa. Quantitative measurement of aggrecanase cleavage requires a neoepitop antibody with specificity for the N-terminus A R G S V I L T . . . appearing upon hydrolysis. The fragment with the newly formed N-terminus is fixed by the neoepitop antibody to a microplate and quantified with an anti-His-tag antibody. In analogy, cleavage by matrix metalloproteinases can be measured with antibodies to neoepitopes appearing upon action of these enzymes. |
Storage And Stability : | Aggrecan interglobular domain is stable until the expiry date given on the label when stored at -70℃. The protein can be kept at -20℃ for several weeks and on ice for several days. Repeated freezing and thawing should be avoided. |
Gene Name : | ACAN aggrecan [ Homo sapiens ] |
Synonyms : | ACAN; aggrecan; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; aggrecan core protein; large aggregating proteoglycan; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein 1; aggrecan 1; aggrecan proteoglyca; Aggrecan core protein 2 |
Gene ID : | 176 |
mRNA Refseq : | NM_001135 |
Protein Refseq : | NP_001126 |
MIM : | 155760 |
UniProt ID : | P16112 |
Chromosome Location : | 15q26.1 |
Function : | extracellular matrix structural constituent; hyaluronic acid binding; protein binding; sugar binding |
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◆ Lysates | ||
ACAN-35HCL | Recombinant Human ACAN cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionOne major challenge is the complex nature of the disease, which involves multiple factors and pathways. Additionally, the high heterogeneity of cartilage tissue and the difficulty in obtaining samples pose challenges for experimental studies. Another challenge is the limited understanding of the molecular mechanisms underlying the degradation and synthesis of ACAN protein and its interactions with other matrix molecules and cell surface receptors.
A high purity level of recombinant protein is desirable as it ensures the protein's integrity and reduces the risk of impurities or contaminants that may affect its function.
ACAN protein is a major component of the extracellular matrix of cartilage and plays a critical role in maintaining its structural integrity. ACAN protein helps to maintain the hydration and swelling properties of cartilage by binding to water molecules through its GAG chains. It also interacts with other matrix molecules and cell surface receptors, which contributes to the mechanical properties of cartilage.
ACAN protein is a large proteoglycan consisting of a core protein and long chains of glycosaminoglycans (GAGs), primarily chondroitin sulfate and keratan sulfate. The core protein is composed of several domains, including a signal peptide, a globular domain, and multiple tandem repeats of a hyaluronan-binding domain (HABD).
The purity level of recombinant protein is typically determined through a combination of different techniques, such as SDS-PAGE, HPLC, and mass spectrometry. The purity level of the protein can vary depending on the production method and the quality control measures in place.
Recombinant ACAN protein is suitable for a variety of applications, including biochemical assays, biophysical studies, and structural biology. The protein can also be used in cell culture experiments and in vitro assays to study its interaction with other proteins and molecules. Additionally, recombinant ACAN protein may have potential applications in tissue engineering and regenerative medicine, where it can be used to create biomaterials for cartilage repair and regeneration.
The endotoxin level of recombinant protein is typically tested using the Limulus amebocyte lysate (LAL) assay. Endotoxins are a type of bacterial cell wall component that can contaminate protein samples, and their presence can affect the function of the protein. A low endotoxin level is desirable for recombinant ACAN protein as it ensures the protein's purity and reduces the risk of contamination. You can select specific recombinant protein endotoxin levels in the inquiry form.
The recommended concentration of recombinant ACAN protein for use in cell culture experiments may vary depending on the specific experimental conditions and cell type used. Typically, a concentration range of 10-100 μg/mL is used.
One potential therapeutic strategy is to use molecules that inhibit the degradation of ACAN protein, such as aggrecanase inhibitors. Another approach is to enhance the synthesis of ACAN protein or its GAG chains, which could be achieved by gene therapy or the use of growth factors. Additionally, tissue engineering approaches that aim to regenerate cartilage by using ACAN protein and other matrix molecules have also been explored.
During the development of osteoarthritis, ACAN protein is degraded by various enzymes, including matrix metalloproteinases and aggrecanases. This degradation results in a loss of cartilage integrity, decreased hydration and swelling properties, and altered mechanical properties of cartilage, which contribute to the development of osteoarthritis.
Customer Reviews (3)
Write a reviewThe technical support team at Creative BioMart went above and beyond to customize the ACAN protein to my specific requirements. Their expertise and professionalism were greatly appreciated.
I was thrilled to find the recombinant ACAN protein on sale at such an affordable price.
I highly recommend Creative BioMart to anyone in need of recombinant proteins. Their products are top-notch and their customer service is exceptional.
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