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HMG-CoA Reductase Activity/Inhibitor Screening Kit

Cat.No. : Kit-2150
Product Overview : HMG-CoA Reductase Activity Assay Kit measures utilization of NADPH, which can be measured by the decrease of absorbance (OD 340 nm). The kit is suitable for measuring activity of purified enzyme and screening inhibitors/activators of HMG-CoA reductase. The limit of detection is below 0.05 mU.
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Size : 100 assays
Applications : Measurement of HMG-CoA reductase activityScreening/studying/characterizing HMG-CoA reductase inhibitors/activators
Storage : Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.HMG-CoA Reductase Assay Buffer: Pre-warm buffer to 37°C before use.HMG-CoA Reductase: Reconstitute in 550 µl of HMG-CoA Reductase Assay Buffer. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice during use.HMG-CoA: Reconstitute in 1.3 ml dH2O, make sure the material is completely dissolved. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice while in use.NADPH: Reconstitute in 440 µl dH2O, make sure the material is completely dissolved. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice while in use.Inhibitor (Atorvastatin, 10 mM): Bring to room temperature before use.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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