Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email:
Home > Products > Assay Kits > Metabolism Kits > NADP/NADPH Assay Kit


"NADP/NADPH" Related Products.
Product Overview:
Detection and Quantification of NADP/NADPH Content.
NADP (Nicotinamide adenine dinucleotide phosphate) is a coenzyme composed of ribosylnicotinamide 5-phosphate (NMN) coupled by pyrophosphate linkage to the 5-phosphate adenosine 2,5-biphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidised (NADP+) and reduced (NADPH). The oxidative phase of the pentose phosphate pathway is the major source of NADPH in cells, producing approxiamtely 60% of the NADPH required. NADPH provides the reducing equivalents for biosynthetic reactions and the oxidation-reduction involved in protecting against the toxicity of ROS, allowing the regeneration of GSH. NADPH is also used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation. NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 570 nm, is proportionate to the NADP+/NADPH concentration in the sample.
Functional Assay
Shipped and store at 4°C for 6 months.
Kit Components:
Component-Volume-Storage1. 96-Well Microplate; 2. Extract I: 30 ml x 1, 4°C; 3. Extract II: 30 ml x 1, 4°C; 4. Reagent I: 10 ml x 1, 4°C; 5. Reagent II: Powder x 1, -20°C; 6. Reagent III: Powder x 1, -20°C; 7. Reagent IV: Powder x 1, 4°C; 8. Reagent V: 1.2 ml x 3, 4°C; 9. Reagent VI: 30 ml x 1, 4°C; 10. Reagent VII: 25 ml x 2, 4°C; 11. Technical Manual: 1 ManualNote: Reagent II: add 3 ml distilled water to dissolve before use, mix, stored at -20°C. Reagent III: add 3 ml distilled water to dissolve before use, mix, stored at 4°C. Reagent IV: add 3 ml distilled water to dissolve before use, mix, stored at 4°C.
Detection method:
Compatible Sample Types:
Urine; Serum; Plasma; Other biological fluids; Tissue Extracts; Cell Lysate

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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