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Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

Cat.No.: Kit-0663
Product Overview: Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
Size: 100 tests
Description: Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
Applications: Functional Studies more details
Storage: Store at -20°C.
Kit Components: Components: Identifier; OxiRed Probe in DMSO: Red; PC Assay Buffer: WM; PC Development Mix (Lyophilised): Green; PC Hydrolysis Enzyme (lyophilized): Purple; PC Standard (10 µmol) (Lyophilised): Yellow
Compatible Sample Types: Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay time: 0h 40m
Sensitivity: >0.2 mM

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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