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Home > Products > Assay Kits > Metabolism Kits > Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

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Product Overview:
Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
100 tests
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
Functional Studies more details
Store at -20°C.
Kit Components:
Components: Identifier; OxiRed Probe in DMSO: Red; PC Assay Buffer: WM; PC Development Mix (Lyophilised): Green; PC Hydrolysis Enzyme (lyophilized): Purple; PC Standard (10 µmol) (Lyophilised): Yellow
Compatible Sample Types:
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay time:
0h 40m
>0.2 mM

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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

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