Recombinant Active Human PAK2 (Y443N) Mutant Protein, GST-tagged

• Cat.No.: PAK2-35H
• Product Overview: Recombinant full-length human PAK2 (Y443N) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
• Source: Insect cell
• Species: Human
• Tag: GST
• Molecular Mass: Approx 95 kDa
• Purity: > 90%
• Stability: One year at -70 centigrade from data of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: PAK2 p21 (RAC1) activated kinase 2 [ Homo sapiens (human) ]
• Official Symbol: PAK2
• Synonyms: PAK2; p21 (RAC1) activated kinase 2; PAK65; PAKgamma; serine/threonine-protein kinase PAK 2; PAK-2; S6/H4 kinase; gamma-PAK; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 2; p58; EC 2.7.11.1
• Gene ID: 5062
• mRNA Refseq: NM_002577
• Protein Refseq: NP_002568
• MIM: 605022
• UniProt ID: Q13177

Reviews

07/27/2021

With this reagent, I can perform a large number of sample determinations in a short period of time.

03/08/2019

Suited for proteomics research, this protein reagent enables efficient screening of large sample volumes.

08/27/2018

Using this reagent, I can complete experiments in a shorter time.

Q&As

What are the upstream regulators of PAK2 signaling?

PAK2 signaling can be regulated by various upstream signaling molecules. Small GTPases, including Rac and Cdc42, are key activators of PAK2. Their activation leads to the recruitment and activation of PAK2 at specific cellular locations. Additionally, growth factors, such as EGF and PDGF, and cytokines can also activate PAK2 through receptor-mediated signaling pathways.

What are the downstream targets of PAK2?

PAK2 phosphorylates and regulates the activity of various downstream targets, affecting multiple cellular processes. Notably, PAK2 can phosphorylate components of the actin cytoskeleton, including LIM kinase and myosin light chain kinase, leading to actin cytoskeleton reorganization and cell motility. PAK2 also phosphorylates transcription factors, such as c-Jun and Elk-1, impacting gene expression and cell proliferation. Moreover, PAK2 can influence cell survival through the regulation of apoptotic factors, such as Bad and Bcl-2 family members. Overall, the downstream targets of PAK2 activation are diverse and depend on the specific cellular context and upstream signals.

What is the function of PAK2 protein in cellular signaling pathways?

PAK2 (p21-activated kinase 2) is a serine/threonine kinase that plays a crucial role in cellular signaling pathways. It is involved in regulating cytoskeletal dynamics, cell migration, cell proliferation, and cell survival. PAK2 is activated by binding to small GTPases, such as Rac and Cdc42, which leads to downstream phosphorylation and activation of various substrates involved in these cellular processes.

What are the mechanisms of PAK2 regulation?

PAK2 is regulated by multiple mechanisms. Its activation is primarily regulated by GTPases, which promote conformational changes and autophosphorylation of PAK2. Additionally, PAK2 activity can be modulated by post-translational modifications, including phosphorylation and ubiquitination. Moreover, PAK2 activity can be influenced by its interaction with scaffolding proteins and other signaling molecules in specific cellular contexts.

What are the substrates of PAK2 protein?

PAK2 phosphorylates a wide range of substrates involved in various cellular processes. Some well-known substrates of PAK2 include LIM kinase, myosin light chain, paxillin, and Bad. These substrates are involved in cytoskeletal dynamics, cell adhesion, cell motility, and apoptosis regulation, highlighting the diverse functional roles of PAK2 in cellular signaling networks.

What is the role of PAK2 protein in cancer progression?

PAK2 has been implicated in cancer progression and metastasis. It promotes tumor cell migration, invasion, and epithelial-mesenchymal transition (EMT) by modulating cytoskeletal dynamics and focal adhesion turnover. PAK2 also influences cancer cell survival and proliferation through its interactions with various oncogenic signaling pathways, such as MAPK and PI3K/Akt. Inhibition of PAK2 has shown potential as a therapeutic strategy to suppress tumor growth and metastasis.

What is the therapeutic potential of targeting PAK2 in disease treatment?

Targeting PAK2 holds therapeutic potential in various diseases, including cancer and neurological disorders. In cancer, inhibition of PAK2 has shown promise in suppressing tumor growth, metastasis, and overcoming drug resistance. Furthermore, PAK2 inhibitors have been explored for the treatment of neurodegenerative diseases, such as Alzheimer's and Parkinson's, due to their involvement in synaptic plasticity and neuronal survival. However, further research is required to optimize the efficacy and safety of PAK2-targeted therapies in clinical settings.