Recombinant Human CD44 protein, GST-tagged

• Cat.No.: CD44-27238TH
• Product Overview: Recombinant Human CD44 (1 a.a. - 699 a.a.) fussed with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 102.63 kDa
• AA Sequence: MDKFWWHAAWGLCLVPLSLAQIDLN ITCRFAGVFHVEKNGRYSISRTEAA DLCKAFNSTLPTMAQMEKALSIGFE TCRYGFIEGHVVIPRIHPNSICAAN NTGVYILTSNTSQYDTYCFNASAPP EEDCTSVTDLPNAFDGPITITIVNR DGTRYVQKGEYRTNPEDIYPSNPTD DDVSSGSSSERSSTSGGYIFYTFST VHPIPDEDSPWITDSTDRIPATSTS SNTISAGWEPNEENEDERDRHLSFS GSGIDDDEDFISSTISTTPRAFDHT KQNQDWTQWNPSHSNPEVLLQTTTR MTDVDRNGTTAYEGNWNPEAHPPLI HHEHHEEEETPHSTSTIQATPSSTT EETATQKEQWFGNRWHEGYRQTPRE DSHSTTGTAAASAHTSHPMQGRTTP SPEDSSWTDFFNPISHPMGRGHQAG RRMDMDSSHSTTLQPTANPNTGLVE DLDRTGPLSMTTQQSNSQSFSTSHE GLEEDKDHPTTSTLTSSNRNDVTGG RRDPNHSEGSTTLLEGYTSHYPHTK ESRTFIPVTSAKTGSFGVTAVTVGD SNSNVNRSLSGDQDTFHPSGGSHTT HGSESDGHSHGSQEGGANTTSGPIR TPQIPEWLIILASLLALALILAVCI AVNSRRRCGQKKKLVINSGNGAVED RKPSGLNGEASKSQEMVHLVNKESS ETPDQFMTADETRNLQNVDMKIGV
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: CD44 CD44 molecule (Indian blood group) [ Homo sapiens ]
• Official Symbol: CD44
• Synonyms: IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; CD44 antigen; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; epican; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1; soluble CD44
• Gene ID: 960
• mRNA Refseq: NM_000610
• Protein Refseq: NP_000601
• MIM: 107269
• UniProt ID: P16070
• Chromosome Location: 11p13
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem
• Function: collagen binding; contributes_to cytokine receptor activity; contributes_to cytokine receptor activity

Reviews

03/08/2022

Using this reagent, I can gain a better understanding of the functionality and regulatory mechanisms of proteins.

04/26/2019

This reagent has long-lasting performance, enduring prolonged experimental use.

03/14/2019

A miraculous reagent driving us towards the frontiers, inviting a glance into an expanded world.

Q&As

What is the role of CD44 in cancer?

CD44 is implicated in cancer progression and metastasis. It promotes tumor cell invasion, migration, and adhesion to the extracellular matrix. CD44 also plays a role in cancer stem cell maintenance and drug resistance. Its overexpression is associated with poor prognosis in various types of cancer.

Can CD44 be targeted for therapeutic purposes?

Targeting CD44 has been explored as a potential therapeutic strategy in cancer and inflammatory diseases. Strategies include blocking CD44-ligand interactions, inhibiting CD44 signaling pathways, and targeting CD44-positive cancer stem cells. However, further research is needed to fully understand the complex roles of CD44 in different disease contexts and to develop effective CD44-targeted therapies.

How is the expression of CD44 regulated?

The expression of CD44 is regulated at multiple levels, including transcriptional control, alternative splicing, and post-translational modifications. Transcription factors such as Sp1 and NF-κB can bind to the CD44 promoter region to regulate its gene expression. Additionally, alternative splicing of CD44 pre-mRNA generates CD44 isoforms with different functions and expression patterns.

How does CD44 contribute to immune regulation?

CD44 is involved in immune cell activation, trafficking, and homing. It participates in the recruitment of leukocytes to sites of inflammation and mediates T cell adhesion and migration. CD44 can also modulate immune responses by interacting with other immune receptors and cytokines, and it is involved in the regulation of T cell activation and differentiation.

How is CD44 involved in immune regulation?

CD44 plays a vital role in immune regulation by participating in immune cell activation, migration, and modulation of T-cell activation and differentiation. CD44 is expressed on various immune cell populations, including T cells, B cells, natural killer cells, and dendritic cells. It serves as an adhesion molecule and facilitates immune cell trafficking to lymphoid tissues and inflammatory sites. CD44 also functions as a co-stimulatory receptor, enhancing T-cell activation and cytokine production upon interaction with its ligands. Moreover, CD44 isoforms have been implicated in the differentiation of T helper cell subsets, such as Th1, Th2, and Th17 cells. These findings highlight the multifaceted role of CD44 in immune regulation and its potential as a therapeutic target for immune-related disorders.

What is the cellular localization of the protein CD44?

CD44 is a transmembrane glycoprotein predominantly localized on the cell surface, specifically in the plasma membrane of various cell types, including epithelial cells, leukocytes, and mesenchymal cells.

What are the signaling pathways associated with CD44?

CD44 can activate various signaling pathways, including PI3K/AKT, MAPK/ERK, and NF-κB pathways. These pathways mediate downstream cellular processes such as cell survival, proliferation, migration, and immune responses. CD44 can also interact with other cell surface receptors, such as receptor tyrosine kinases, to modulate their signaling activities.