Recombinant Human Fibroblast Growth Factor

• Cat.No.: FGFR2-102H
• Product Overview: Recombinant Human Fibroblast Growth Factor-2 (FGF-2) produced inSf9insect cells is a single, glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton. The FGF-basic is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: FGFR2-102H
• Description: This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
• Source: Baculovirus.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile.
• Physical Appearance: Sterile Filtered liquid formulation.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The sterile protein solution (0.5 mg/ml) contains 20 mM Tris pH=7.9, 100 mM KCl, 1 mM DTT and 20% glycerol.
• Specific Activity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2 x 106 Units/mg.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.
• Stability: Fibroblast Growth Factor-basic although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: FGFR2 fibroblast growth factor receptor 2 [ Homo sapiens ]
• Synonyms: FGFR2; fibroblast growth factor receptor 2; BEK; JWS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM; FLJ98662; FGFR2; KSAR; FGFR-2; KSAM; OTTHUMP00000020621; OTTHUMP00000020629; BEK fibroblast growth factor receptor; CD332 antigen; FGF receptor; Jackson-Weiss syndrome; Keratinocyte growth factor receptor 2; bacteria-expressed kinase; craniofacial dysostosis 1; fibroblast growth factor receptor 2; hydroxyaryl-protein kinase; keratinocyte growth factor receptor; protein tyrosine kinase, receptor like 14; soluble FGFR4 variant 4
• Gene ID: 2263
• mRNA Refseq: NM_000141
• Protein Refseq: NP_000132
• UniProt ID: P21802
• Chromosome Location: 10q26
• MIM: 176943
• Pathway: Endocytosis; MAPK signaling pathway; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Signaling by FGFR
• Function: ATP binding; fibroblast growth factor receptor activity; heparin binding; nucleotide binding; protein binding; receptor activity; transferase activity

Reviews

08/24/2017

The FGFR2 protein is an outstanding product that possesses exceptional quality, making it an ideal choice for my experimental needs.

03/05/2017

What sets FGFR2 apart is the manufacturer's unparalleled technical support.

09/13/2016

The versatility of the FGFR2 protein makes it a valuable asset in my research.

Q&As

How is FGFR2 linked to developmental disorders?

Mutations in FGFR2 are associated with various developmental disorders, impacting the development of the skull, limbs, and other structures.

Can FGFR2 be targeted for personalized cancer therapy?

Yes, targeting FGFR2 mutations allows for more personalized and precise therapeutic approaches in cancer treatment.

Are there clinical trials involving FGFR2 inhibitors?

Yes, numerous clinical trials are underway to evaluate the efficacy of FGFR2 inhibitors in treating various cancers and developmental disorders.

How does FGFR2 impact wound healing?

FGFR2 is involved in the regulation of cell migration and tissue repair, contributing to the process of wound healing.

Are there any side effects associated with FGFR2 inhibitors?

Common side effects of FGFR2 inhibitors include fatigue, nausea, and hypertension, which are being studied for better management.