"Dkk2" Related Products

Recombinant Mouse Dkk2 protein, His-tagged

Cat.No.: Dkk2-194M
Product Overview: Recombinant Mouse Dkk2(Ser26-Ile259) fused with Leu35Pro substitution and His tag at C-terminal was expressed in E. coli.
Description: Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators. Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain mediates Dkk-2 binding activities, and its interaction with LRP beta propellers has been mapped. The 226 aa, ~35 kDa mature mouse Dkk-2 shares 99%, 96%, 96%, 96% and 94% aa identity with rat, human, dog, horse and cow Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos. Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6. When LRP6 is over-expressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling. However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes. Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling. In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic. Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye, although it is expressed as early as periimplantation in mice. Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite down-regulating Wnt antagonism in both cases. Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition. In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density. Dkk-2-deficient mice are blind, exhibiting faulty differentiation of corneal epithelium and ectopic blood vessels in the periocular mesenchyme.
Source: E. coli
Species: Mouse
Tag : His
Predicted N Terminal: Ser26
Form: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity: Measured in a competitive binding assay. When Recombinant Mouse Kremen‑1 is immobilized at 4 µg/mL (100 µL/well), Recombinant Mouse Dkk-2 inhibits 50% binding of biotinylated Recombinant Human Dkk-1 (200 ng/mL) at the concentration range of 0.25-1.5 µg/mL.
Molecular Mass: Predicted Molecular Mass: 27 kDa
SDS-PAGE: 29-35 kDa, reducing conditions
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: DKK2 dickkopf 2 homolog (Xenopus laevis) [ Homo sapiens ]
Official Symbol: Dkk2
Synonyms: DKK2; dickkopf 2 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 2; dickkopf-related protein 2; hDkk-2; dickkopf-2; dickkopf homolog 2; dickkopf related protein-2; DKK-2;
Gene ID: 27123
mRNA Refseq: NM_014421
Protein Refseq: NP_055236
MIM: 605415
UniProt ID: Q9UBU2

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.