LDHB
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Official Full Name
lactate dehydrogenase B
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Overview
This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. -
Synonyms
LDHB; lactate dehydrogenase B; L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; LDH-B; LDH-H; LDHBD; TRG-5;
- Native Proteins
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- Protein Pre-coupled Magnetic Beads
- Assay Kits
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- Wheat Germ
- GST
- His
- His (Fc)
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- N/A
- Background
- Quality Guarantee
- Case Study
- Involved Pathway
- Protein Function
- Interacting Protein
- LDHB Related Research Area
What is LDHB protein?
LDHB (lactate dehydrogenase B) gene is a protein coding gene which situated on the short arm of chromosome 12 at locus 12p12. This gene encodes the B subunit of lactate dehydrogenase enzyme called LDHB, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. This protein is consisted of 334 amino acids and is approximately 36.6 kDa.
What is the Function of LDHB protein?
Lactate dehydrogenase B (LDHB) plays an important role in cellular energy metabolism and lactic acid balance, and is involved in the glycolysis pathway, which can convert glucose into pyruvate and lactic acid. By converting lactic acid to pyruvate, LDHB can participate in the cell's oxidative phosphorylation process, producing more ATP energy for the cell to use. In addition, LDHB is also involved in the elimination and transport of lactic acid, maintaining the balance of lactic acid inside and outside the cell.
LDHB Related Signaling Pathway
LDHB is mainly involved in the glycolysis pathway and its function is to convert glucose into pyruvate and lactic acid. However, in addition to these metabolic functions, studies have shown that LDHB also plays an important role in multiple signaling pathways. LDHB can activate VEGF/VEGFR2 signaling to regulate tumor angiogenesis. LDHB also regulates the NFKB signaling pathway through the mitochondrial autophagy pathway.
Fig1. The "Goldilocks principle" in regulating glycolysis and differentiation. (Xuyong Chen, 2023)
LDHB Related Diseases
Mutations in the LDHB gene have been linked to a variety of diseases, including:
Lactate dehydrogenase B deficiency (LDHBD): This is a rare genetic disorder in which patients lack LDHB enzyme activity, resulting in elevated levels of lactic acid and pyruvate. Metabolic diseases: LDHB is involved in the process of glucose metabolism and energy production, and abnormal expression of LDHB is associated with type 2 diabetes, obesity and metabolic syndrome. Cardiovascular diseases: LDHB is upregulated in myocardial ischemia-reperfusion injury and may be involved in cardiac cell death and cardiac function restoration. At the same time, LDHB is highly expressed in a variety of tumors, including breast cancer, lung cancer, and colon cancer.
Bioapplications of LDHB
LDHB has also been studied as a potential therapeutic target for cancer treatment. Inhibition of LDHB activity has been shown to impair tumor growth in preclinical studies. Targeting LDHB may help to disrupt the metabolism of cancer cells and sensitize them to other therapies.
High Purity
Fig1. SDS-PAGE (Ldhb-1817M) (PROTOCOL for western blot)
Case Study 1: Suxing Jin, 2023
Fig1. The expressions of LDHA and LDHB relative to α-tubulin in SKOV-3 cells after treatment with CDDP, MDP, DDP (0.6 μM containing 0.6‰ DMSO), and DCF (1.2 μM containing 0.1‰ DMSO), respectively, for 36h.
Case Study 2: Manos Vlasiou, 2023
Fig2. Effect of tucatinib on tetramer formation and activity of LDH-B. Equal amounts of LDH-B (5 ng) were incubated with increasing concentrations of either tucatinib for 30 min, followed by loading onto 10% native (non-reducing) polyacrylamide gels.
Fig2. Schematic illustration of the direct effects of CaLac on anaerobic glycolysis under hypoxia. (Keun-Yeong Jeong, 2021)
LDHB involved in several pathways and played different roles in them. We selected most pathways LDHB participated on our site, such as Glycolysis / Gluconeogenesis, Cysteine and methionine metabolism, Pyruvate metabolism, which may be useful for your reference. Also, other proteins which involved in the same pathway with LDHB were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Glycolysis / Gluconeogenesis | ALDH9A1;ALDH9A1A.1;LDHBA;ADH1;GALM;ALDOCA;PDHA2;AKR1A1;PFKM |
| Cysteine and methionine metabolism | BCAT2;SDS;GOT2B;GSS;AHCY;LDHA;MDH1AA;IL4I1;BHMT |
| Pyruvate metabolism | PDHA1A;GRHPRB;LDHAL6B;ACYP2;PCK2;ALDH9A1;DLDH;LDHA;LDHB |
| Propanoate metabolism | LDHBA;SUCLG2;ACADM;ECHDC1;HIBCH;LDHBB;ACAT1;HADHAB;PCCA |
| Metabolic pathways | ACSM4;CYP2C45;PLA2G4AA;PYGM;NTPCR;ATP6V0E1;SDHDB;PCYT2;RRM2 |
LDHB has several biochemical functions, for example, L-lactate dehydrogenase activity, NAD binding, identical protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by LDHB itself. We selected most functions LDHB had, and list some proteins which have the same functions with LDHB. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| L-lactate dehydrogenase activity | LDHC;LDHA;LDHB;LDHBB;LDHAL6B;LDHAL6A;LDHBA |
| NAD binding | SORD;DLD;HSD3B4;HPGD;IDH2;LDHA;TESCA;CTBP1;UGDH |
| identical protein binding | CD28;KCNIP2;CCDC155;LCK;DIAP1;C14orf166;HACL1;FADD;PON2 |
| kinase binding | WWC3;TRADD;PER3;GFAP;JAKMIP3;JAKMIP2;PRKCD;CAB39;UBQLN1 |
| protein binding | TULP1;RIPPLY1;ARAP1;BCS1L;CHRNB2;SMARCC1;SPAST;POLD3;MED25 |
LDHB has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with LDHB here. Most of them are supplied by our site. Hope this information will be useful for your research of LDHB.
LDHA; PEX5; VCAM1; LRRK2; TERF1; RAC3; YWHAZ; TBK1; LDHA; artM4
- Q&As
- Reviews
Q&As (5)
Ask a questionYes, LDHB dysregulation is associated with heart conditions like myocardial infarction (heart attack) and heart failure.
While LDHB-related diseases can affect anyone, some genetic predispositions may heighten the risk in certain ethnic groups.
It plays a role in various physiological processes and has implications in disease states.
In certain cancers, elevated LDHB levels promote tumor growth by supporting the Warburg effect, where cancer cells use glycolysis even in the presence of oxygen.
Targeting LDHB shows promise in inhibiting cancer growth, and there's ongoing research in developing drugs that target this enzyme.
Customer Reviews (3)
Write a reviewI highly endorse the utilization of the LDHB Protein for researchers seeking a superior protein solution that not only meets their experimental needs but also benefits from the exceptional technical support provided by the manufacturer.
Their expertise has proven instrumental in overcoming obstacles and has greatly contributed to the success of my research endeavors.
Their team of experts has been extremely helpful in addressing any concerns or queries I encountered during my experiments.
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