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PHF8

Official Full Name

PHD finger protein 8
Overview

The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene.
Synonyms

PHF8; PHD finger protein 8; histone lysine demethylase PHF8; JHDM1F; jumonji C domain containing histone demethylase 1F; KIAA1111; ZNF422; jumonji C domain-containing histone demethylase 1F; MRXSSD; DKFZp686E0868;

Type

Recombinant Proteins
Protein Pre-coupled Magnetic Beads
Assay Kits

Species

Human
Mouse
Zebrafish

Source

E.coli
HEK293
Mammalian Cell
Sf9 Insect Cell

Tag

Flag
His
His (Fc)
Avi
N/A
No tag

Species	Cat.#	Product name	Source (Host)	Tag	Protein Length	Price
Human	PHF8-61H	Active Recombinant Human PHF8, FLAG-tagged	Sf9 Insect Cell	Flag		Inquiry
Human	PHF8-63H	Recombinant Human PHF8, GST-tagged	E.coli	N/A		Inquiry
Human	PHF8-62H	Recombinant Human PHF8, GST-tagged	E.coli	N/A		Inquiry
Human	PHF8-0538H	Recombinant Human PHF8 Protein (A38-N483), His tagged	E.coli	His	A38-N483	Inquiry
Human	PHF8-0537H	Recombinant Human PHF8 Protein (A38-N483), Tag Free	E.coli	No tag	A38-N483	Inquiry
Mouse	PHF8-12733M	Recombinant Mouse PHF8 Protein	Mammalian Cell	His		Inquiry
Mouse	Phf8-751M	Recombinant Mouse Phf8 Protein, His-tagged	E.coli	His	Phe195~Lys351	$298 / 10ug
Mouse	PHF8-6695M-B	Recombinant Mouse PHF8 Protein Pre-coupled Magnetic Beads	HEK293			Inquiry
Mouse	PHF8-6695M	Recombinant Mouse PHF8 Protein, His (Fc)-Avi-tagged	HEK293	His (Fc)-Avi		Inquiry
Zebrafish	PHF8-7972Z	Recombinant Zebrafish PHF8	Mammalian Cell	His		Inquiry
	Kit-1851	PHF8 (KDM7B) Homogeneous Assay Kit		N/A		Inquiry

Involved Pathway
Protein Function
Interacting Protein
PHF8 Related Articles

PHF8 involved in several pathways and played different roles in them. We selected most pathways PHF8 participated on our site, such as Cell Cycle, Cell Cycle, Mitotic, Chromatin modifying enzymes, which may be useful for your reference. Also, other proteins which involved in the same pathway with PHF8 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name	Pathway Related Protein
Cell Cycle	LIN54;RAD50;CENPW;DSN1;FOXM1;ENSAB;UBE2V2;RBL1;ATM
Cell Cycle, Mitotic	MAPRE1B;H3F3B.1;RCC2;SMC4;CKS1B;FGFR1OP;FOXM1;NDE1;H3F3D
Chromatin modifying enzymes	KDM6B;RBBP4;TADA1;SMARCE1;KDM4A;MTA2;CHD4A;CSRP2BP;KDM2B
Chromatin organization	PRDM16;SMARCA2;KDM2A;ATF7IP;MSL1A;CSRP2BP;MCRS1;BRPF1;ELP3
Condensation of Prophase Chromosomes	H3F3D;SMC4;SET;NCAPH2;NCAPD3;H3F3B.1;PHF8;SETB;MCPH1
HDMs demethylate histones	KDM6AL;KDM2AA;KDM2A;KDM4C;KDM4A;KDM1B;JHDM1D;KDM5BA;PHF2
M Phase	DULLARD;CENPH;NSL1;SGOL2;NIPBL;CTDNEP1A;BLZF1;KIF18A;USO1
Mitotic Prophase	TMEM188;NCAPD3;MCPH1;GORASP2;NCAPH2;NEK6;VRK2;SETA;ARPP19A

PHF8 has several biochemical functions, for example, chromatin binding, histone demethylase activity, histone demethylase activity (H3-K27 specific). Some of the functions are cooperated with other proteins, some of the functions could acted by PHF8 itself. We selected most functions PHF8 had, and list some proteins which have the same functions with PHF8. You can find most of the proteins on our site.

Function	Related Protein
chromatin binding	PRDM13;PRKCBP1L;TOX2;ANKRD17;CREBBP;KDM5A;MLL1;NFIA;NEUROD1
histone demethylase activity	PHF8;KDM1A;KDM4C;KDM2B
histone demethylase activity (H3-K27 specific)	KDM6A;KDM6B;KDM7AB;KDM6BB;PHF8;JHDM1D
histone demethylase activity (H3-K36 specific)	JMJD5;JHDM1D;KDM8;KDM4A;PHF8;KDM2A;KDM2B;C14orf169
histone demethylase activity (H3-K9 specific)	PHF8;KDM4C;PHF2;KDM4D;KDM7AB;KDM1A;JHDM1D
histone demethylase activity (H4-K20 specific)	HIST1H4A;HIST1H4I;HIST1H4B;HIST1H4F;HIST1H4C;HIST1H4J;HIST1H4K;HIST2H4A;JHDM1D
iron ion binding	CYP2Y3;NOS3;CYP2K19;CYP51;CYP2C70;CALR;NOS2B;P4HA3;CYP4Z1
methylated histone binding	KAT8;L3MBTL1;LOXL2;DPPA3;PHF1;CAPNS2;LRWD1;CHD1;CBX7
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors	TYW5;C14orf169;EGLN2;ALKBH4;KDM5C;KDM5BA;JHDM1D;KDM5A;HIF1AN
protein binding	CD200R1;LC3;BAG1;MTOR;VEGFB;ZFYVE1;CCDC59;STXBP2;CCNDBP1
zinc ion binding	VPS29;LHX1B;ADAM15;PHF20B;MLLT6;MARCH1;MARCH4;SP140L;ADAM21

PHF8 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PHF8 here. Most of them are supplied by our site. Hope this information will be useful for your research of PHF8.

Dlg4; PML-RAR; h3_peptide_2-18; rarb_human_gene; H2AFY; CENPB; EIF6; HIST1H3D; MRPS35; MRPS15; XPC; TRIM26; TRIP12; PLEC; HIST2H2BE; DNTTIP2; RIF1; BRAT1; NOL8; MB21D1

Shi, G; Wu, M; et al. PHD Finger Protein 2 (PHF2) Represses Ribosomal RNA Gene Transcription by Antagonizing PHF Finger Protein 8 (PHF8) and Recruiting Methyltransferase SUV39H1. JOURNAL OF BIOLOGICAL CHEMISTRY 289:29691-29700(2014).

Lubula, MY; Poplawaski, A; et al. Crystallization and preliminary X-ray diffraction analysis of the BRPF1 bromodomain in complex with its H2AK5ac and H4K12ac histone-peptide ligands. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70:1389-1393(2014).