ANAPC4
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Official Full Name
anaphase promoting complex subunit 4
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Overview
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. -
Synonyms
ANAPC4; anaphase promoting complex subunit 4; anaphase-promoting complex subunit 4; APC4; ANAPC 4; APC 4; APC Complex Protein CUT20; APC4_HUMAN; CUT20; Cyclosome subunit 4; LID1; OTTHUMP00000123382; OTTHUMP00000218479;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Human
- Rhesus Macaque
- Zebrafish
- HEK293
- Mammalian Cell
- Wheat Germ
- GST
- His
- His (Fc)
- Avi
- N/A
Species | Cat.# | Product name | Source (Host) | Tag | Protein Length | Price |
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Human | ANAPC4-545H | Recombinant Human ANAPC4 protein, GST-tagged | Wheat Germ | GST | ||
Human | ANAPC4-73HCL | Recombinant Human ANAPC4 cell lysate | N/A | |||
Rhesus Macaque | ANAPC4-323R | Recombinant Rhesus monkey ANAPC4 Protein, His-tagged | Mammalian Cell | His | ||
Rhesus Macaque | ANAPC4-151R-B | Recombinant Rhesus Macaque ANAPC4 Protein Pre-coupled Magnetic Beads | HEK293 | |||
Rhesus Macaque | ANAPC4-151R | Recombinant Rhesus Macaque ANAPC4 Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi | ||
Zebrafish | ANAPC4-1887Z | Recombinant Zebrafish ANAPC4 | Mammalian Cell | His |
- Involved Pathway
- Protein Function
- Interacting Protein
ANAPC4 involved in several pathways and played different roles in them. We selected most pathways ANAPC4 participated on our site, such as Cell cycle, Oocyte meiosis, Ubiquitin mediated proteolysis, which may be useful for your reference. Also, other proteins which involved in the same pathway with ANAPC4 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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Cell cycle | WEE1;NIPBL;GOLGA2;CEP135;PIANP;WRAP53;PMF1;SKP2;CDC14AA |
Oocyte meiosis | CAMK2B1;PRKACBA;CDC20;CTH1;CAMK2G1;CALM3A;PPP1R2P9;RPS6KAL;CDC16 |
Ubiquitin mediated proteolysis | TRIP12;CUL4A;BRCA1;PML;UBE2KB;ITCH;UBE2IA;FBXW7;PRPF19 |
Progesterone-mediated oocyte maturation | MAPK14B;ANAPC5;INS2;ARAF;ANAPC1;CPEB1A;ADCY2B;CPEB1B;CPEB4 |
HTLV-I infection | POLE2;SLC2A1;MSX1;WNT1;RRAS;FZD1;KRAS;MAP2K4;PDGFRA |
ANAPC4 has several biochemical functions, for example, protein binding, protein phosphatase binding, ubiquitin-protein transferase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by ANAPC4 itself. We selected most functions ANAPC4 had, and list some proteins which have the same functions with ANAPC4. You can find most of the proteins on our site.
Function | Related Protein |
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protein binding | FGF1;TEKT3;MSTN;TACR3;NDOR1;PEX2;HJURP;HSPA4;BTC |
protein phosphatase binding | MAP3K5;CSK;CDKN1B;ERBB2;DRD1A;CDH5;ANAPC5;PPP1R3C;JAK1 |
ubiquitin-protein transferase activity | TRIM68;MDM2;LTN1;TRAF4;PEX12;RNF187;ARIH1L;RNF25;MARCH5 |
ANAPC4 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ANAPC4 here. Most of them are supplied by our site. Hope this information will be useful for your research of ANAPC4.
CDC27; BUB1B; Cdc23; Cdc16; Cdc26; CDC20
- Q&As
- Reviews
Q&As (21)
Ask a questionCurrently, there are no specific diseases or conditions directly associated with ANAPC4 mutations. However, mutations in other subunits of the APC/C complex have been linked to developmental disorders, intellectual disabilities, and various types of cancer.
Yes, ANAPC4 has been implicated in other cellular processes beyond cell cycle regulation. It has been found to have roles in DNA repair, chromatin remodeling, transcriptional regulation, and centrosome duplication.
Yes, ANAPC4 has been implicated in the regulation of centrosome duplication, which is essential for proper mitotic spindle formation. It interacts with proteins involved in centrosome duplication, such as Plk1, and its dysregulation can lead to abnormal centrosome duplication and mitotic defects.
Various experimental techniques are used to study ANAPC4 protein, including biochemical analyses, immunoprecipitation, immunofluorescence, genetic approaches (gene knockout or knockdown), and cell cycle analysis. Additionally, structural studies, such as X-ray crystallography or cryo-electron microscopy, can provide insights into the protein's structure-function relationship.
ANAPC4 is a crucial component of the APC/C complex, which plays a central role in cell cycle regulation. During mitosis, the APC/C complex is activated and targets specific proteins for degradation through ubiquitin-mediated proteolysis. ANAPC4, along with other subunits of the APC/C complex, helps recognize and bind to specific target proteins, marking them for degradation and allowing proper progression through the cell cycle.
Currently, there are no specific inhibitors or modulators specifically targeting ANAPC4. However, inhibitors of the APC/C complex, such as proTAME, have been developed and shown promising effects in preclinical studies. These inhibitors may indirectly affect ANAPC4 activity within the APC/C complex.
Yes, ANAPC4 is highly conserved across different species, from yeast to humans. This conservation suggests its importance in fundamental cellular processes and highlights its essential role in cell cycle regulation and other cellular functions.
There are currently no known clinical trials specifically targeting ANAPC4 or the APC/C complex. However, targeting the APC/C complex as a whole has been explored in preclinical studies and has shown potential for therapeutic interventions in cancer treatment.
Yes, mutations in ANAPC4 and other subunits of the APC/C complex have been found in various types of cancer. Dysregulation of the APC/C complex can lead to abnormal cell cycle progression, genomic instability, and unchecked cell growth, which are all hallmarks of cancer development.
ANAPC4, as part of the APC/C complex, has been found to interact with proteins involved in DNA repair, such as BRCA2 and RAD51. These interactions suggest a role for ANAPC4 in regulating DNA repair processes, particularly homologous recombination.
Currently, there is no specific genetic disorder that has been directly linked to ANAPC4 mutations. However, mutations in other subunits of the APC/C complex have been associated with developmental disorders and intellectual disabilities.
The activity of ANAPC4 is tightly regulated through multiple mechanisms. Its expression is regulated during the cell cycle, and its association with other subunits of the APC/C complex is crucial for its activity. Additionally, post-translational modifications, such as phosphorylation, can modulate ANAPC4 function.
Mutations or dysregulation of APC/C complex subunits, including ANAPC4, have been implicated in various human diseases and disorders, including certain types of cancer, neurodegenerative diseases, and developmental disorders.
Yes, ANAPC4 can undergo post-translational modifications, including phosphorylation. Phosphorylation of ANAPC4 can regulate its activity and interactions within the APC/C complex.
Yes, ANAPC4 can interact with other cellular pathways and signaling networks. It has been implicated in DNA repair processes, such as homologous recombination, and can also affect transcriptional regulation through interactions with transcription factors or co-regulators. Further studies are needed to fully understand these interactions.
Currently, ANAPC4 is not widely used as a diagnostic marker for specific diseases. However, further research may uncover its potential as a diagnostic marker or therapeutic target in certain conditions.
Targeting ANAPC4 directly as a therapeutic intervention is challenging due to its essential role in the APC/C complex and its involvement in multiple cellular processes. However, targeting the APC/C complex as a whole has been explored as a potential therapeutic strategy, particularly in the context of cancer treatment.
Yes, ANAPC4 is essential for cell viability and proper cell cycle progression. The loss or dysfunction of ANAPC4 can lead to cell cycle defects and impaired chromosome segregation.
Yes, ANAPC4 has been shown to interact with transcription factors and co-regulators, suggesting a role in transcriptional regulation. It may modulate gene expression by influencing the stability and activity of transcription factors or by regulating the turnover of specific proteins involved in transcription.
Currently, there are no specific inhibitors or drugs targeting ANAPC4. However, targeting the APC/C complex as a whole has been explored as a potential therapeutic strategy in certain cancers.
While the primary known function of ANAPC4 is its role in the cell cycle regulation, emerging evidence suggests potential roles in other cellular processes, such as DNA repair and transcriptional regulation.
Customer Reviews (5)
Write a reviewThe manufacturer's team of experts possesses a deep understanding of the ANAPC4 protein and its associated protocols.
One of the key advantages of using ANAPC4 protein in trials is its high quality.
This high-quality ANAPC4 protein provides researchers with confidence in obtaining accurate and reliable data during their investigations
The manufacturer ensures that the protein is produced with utmost purity and integrity, minimizing the risk of experimental artifacts or compromised results.
In conclusion, using ANAPC4 protein in trials offers distinct advantages for researchers studying cell cycle regulation.
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