Thrombin Generation Assay Kit

• Cat.No.: Kit-0982
• Product Overview: The TGA kit is an assay system for determination of thrombin generation over time in platelet poor or platelet rich plasma (PPP or PRP) upon activation of the clotting cascade by micelles of negatively charged phospholipids containing different amounts of human tissue factor and CaCl2. The kit can be used to monitor hemophiliacs during inhibitor bypassing therapy, to monitor anticoagulation therapy, to calculate INR values for patients and to determine states of bleeding disorders or thrombophilia as well as the activity of circulating micro particles. This broad range of applications is possible by providing different tissue factor concentrations and by monitoring the whole kinetic of thrombin generation during initiation, amplification and down regulation of thrombin formation. TGA is therefore a universal assay kit for analyzing and monitoring the function of the haemostatic system.

Specification

• Storage: Reagent RT*(20...25°C) +2…8°C -20°C
  TGA substrate (SUB) 1 week 1 month 6 months
  TGA buffer (BUF) 8 hours 1 week 1 month
  TGA thrombin calibrator (CAL) 8 hours 1 week 6 months
  TGA reagent B (RB), C Low
  (RCL) , High (RCH), D (RD) 8 hours 1 week 6 months
  TGA control high (CH) 4 hours 8 hours 1 month
  TGA control low (CL) 4 hours 8 hours 1 month
  Avoid contamination by micro-organisms.
  Plasmas should be frozen only once; during storage, the vials should be tightly capped.
  Stability of the sample material: * room temperature
  Sample material RT* (20...25°C) +2…8°C -20°C
  PPP, PRP and PFP Plasma 2 hours 4 hours 1 month
  An immediate centrifugation after blood withdrawal is recommended.
  Further we recommend an immediate shock freezing of the centrifuged samples.
  Attention! The frozen samples should be stored in a constant environment - avoid exposing the samples to variations in temperature.
  Before transportation we recommend to centrifuge and prepare the samples.
• Size: 3x16 tests