MMP-9 Inhibitor Screening Kit

• Cat.No.: Kit-2130
• Product Overview: MMP-9 Inhibitor Screening Kit provides a quick and sensitive way for screening, studying and characterizing potential inhibitors of MMP-9. In this assay, MMP-9 hydrolyzes a FRET-based MMP-9 substrate and releases the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm.

Specification

• Size: 100 assays
• Applications: Screening/studying/characterizing potential MMP-9 inhibitors
• Storage: Store the kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read the entire protocol before performing the assay.MMP-9 Assay Buffer and MMP-9 Substrate: Bring to room temperature before use.MMP-9: Reconstitute with 110 µl pre-chilled 30% Glycerol solution (in dH2O). Keep on ice until it completely dissolves. Store the reconstituted MMP-9 in aliquots at -20°C. Prevent repeated freeze thaw cycles.Inhibitor NNGH (2 mM): Aliquot and store at -20°C.