Active Recombinant Cynomolgus Monkey MMP9 Protein (Ala20-Asp707)

• Cat.No.: MMP9-13C
• Product Overview: Recombinant Cynomolgus Monkey MMP9 Protein (Ala20-Asp707) without tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: Matrix metalloproteinase 9 (MMP-9), also known as gelatinase B, is a member of the MMP zinc-dependent family of endopeptidases. It cleaves and degrades a variety of targets including important extracellular matrix (ECM) proteins: gelatin, collagen, and elastin, as well as chemokines and extracellular domain plasma membrane proteins. MMP-9 is synthesized and secreted by several cells including neutrophils, macrophages, fibroblasts, and endothelial cells. The monomeric MMP-9 protein is composed of several distinct domains including a signal sequence, a pro-domain which is cleaved upon activation, and a catalytic domain at the n-terminus followed by a hinge region and the c-terminal hemopexin-like domains that contribute to substrate recognition and specificity. The catalytic domain contains fibronectin type II domains, an active site, and a zinc binding site. MMP-9 can exist as a monomer, disulfide-linked homodimer, or heterodimer in complex with lipocalin-2. MMP-9 activity is regulated at several levels via transcription, post-transcription, translation, secretion, activation, and inhibition. As MMP-9 is involved in ECM remodeling and membrane protein cleavage, it has been widely associated to play a role in several diseases including cancers, autoimmune, and cardiovascular diseases. MMP-9 is consequently an important target of interest for inhibition. Additionally, it has been found to be a potential biomarker for many types of cancer including pancreatic, osteosarcoma, lung, ovarian, and breast.
• Source: CHO
• Species: Cynomolgus Monkey
• Form: Proform
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >750 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 76 kDa
  SDS-PAGE: 83-95 kDa, under reducing conditions
• N-terminal Sequence Analysis: Ala20
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: MMP9 matrix metallopeptidase 9 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: MMP9 matrix metallopeptidase 9 [ Macaca fascicularis (crab-eating macaque) ]
• Synonyms: MMP9; matrix metallopeptidase 9; matrix metalloproteinase-9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Gene ID: 102117693
• mRNA Refseq: XM_005569214
• Protein Refseq: XP_005569271