Protein Characterization

Analytical Protein Characterization ensures the identity, purity, structural and conformational integrity and activity of a protein. The precise amino acid sequence, molecular weight, charge variances, glycosylation, aggregation level, and oxidation level are all key components of thorough characterization of recombinant protein.

Creative BioMart performs a number of routine protein analyses throughout all project stages to understand the heterogeneity, impurity profile, and potency of a recombinant protein destined for pharmaceutical use. We have developed the platforms and analytical methods to pursue a comprehensive chemical, physical, and conformational characterization.

Preparation and submission of protein samples for

  • Protein identity, purity and impurities
  • Charge isoforms
  • Oxidation
  • Aggregation analysis
  • Amino acid analysis
  • Peptide mapping
  • Glycan analysis and profiling

Platform and Analytical Techniques

Protein Identity, Purity and Impurities
  • SDS electrophoresis
  • Analytical Size-Exclusion Chromatography
  • Chip based Protein Electrophoresis Assays
  • Caplillary electrophoresis
  • UV detection
  • HPLC
Charge Isoform
  • Gel Isoelectric Focusing
  • Capillary Isoelectric Focusing
  • Whole-Column Isoelectric Focuing
  • OFFGEL electrophoresis
  • Ion Exchange Column-HPLC
Aggregation analysis
  • SDS PAGE
  • Chip-based Protein Electrphoresis
  • Size Exclusion HPLC
  • Light Scattering
Oxidation
  • HPLC-LC/UV
  • Reversed-Phase LC/MS
  • Enzymatic digestion + LC/MS
 
Amino Acid Analysis
  • OPA derivitization + RP-LC/UV
  • FMOC derivitization + RP-LC/UV
  • Derivitization + RP-LC/MS
Peptide Mapping
  • RP-LC/UV
  • RP-LC/MS
  • Capillary electrophoresis detection by DAD (diode array detection) or MS
Glycan Profiling
  • HPLC/fluorescence detection
  • CE/fluorescence
  • MALDI-TOF/MS
  • LC/MS
  • HPLC/pulsed amperiometric detector

To find out more about protein characterization services for biologics, contact us immediately.

Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information.

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