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Your Position:  Home >> Services >>Protein Characterization

Protein Characterization


Analytical Protein Characterization ensures the identity, purity, structural and conformational integrity and activity of a protein. The precise amino acid sequence, molecular weight, charge variances, glycosylation, aggregation level, and oxidation level are all key components of thorough characterization of recombinant protein.

Creative BioMart performs a number of routine protein analyses throughout all project stages to understand the heterogeneity, impurity profile, and potency of a recombinant protein destined for pharmaceutical use. We have developed the platforms and analytical methods to pursue a comprehensive chemical, physical, and conformational characterization.

Preparation and submission of protein samples for

  • Protein identity, purity and impurities
  • Charge isoforms
  • Oxidation
  • Aggregation analysis
  • Amino acid analysis
  • Peptide mapping
  • Glycan analysis and profiling



Platform and Analytical Techniques

Protein Identity, Purity and Impurities
  • SDS electrophoresis
  • Analytical Size-Exclusion Chromatography
  • Chip based Protein Electrophoresis Assays
  • Caplillary electrophoresis
  • UV detection
  • HPLC
Charge Isoform
  • Gel Isoelectric Focusing
  • Capillary Isoelectric Focusing
  • Whole-Column Isoelectric Focuing
  • OFFGEL electrophoresis
  • Ion Exchange Column-HPLC
Aggregation analysis
  • Chip-based Protein Electrphoresis
  • Size Exclusion HPLC
  • Light Scattering
  • Reversed-Phase LC/MS
  • Enzymatic digestion + LC/MS

Amino Acid Analysis
  • OPA derivitization + RP-LC/UV
  • FMOC derivitization + RP-LC/UV
  • Derivitization + RP-LC/MS
Peptide Mapping
  • RP-LC/UV
  • RP-LC/MS
  • Capillary electrophoresis detection by DAD (diode array detection) or MS
Glycan Profiling
  • HPLC/fluorescence detection
  • CE/fluorescence
  • LC/MS
  • HPLC/pulsed amperiometric detector

To find out more about protein characterization services for biologics, contact us immediately.

Welcome to contact us or send an email to for project quotations and more detailed information.
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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