Yeast Expression Systems

As a well-defined and economical eukaryotic expression system for both secretory and intracellular expression, yeast protein expression system is perfectly suitable for large-scale manufacture of recombinant proteins. Creative BioMart has developed yeast expression vectors that are optimized for the efficient production of heterologous proteins. We have also a few strong signal sequences to ensure the highest secretion level.

All of below advantages make yeast expression system an ideal host for producing large quantities of properly folded proteins in a short period of time:

  • High yield
  • High productivity
  • Genetics can be easily manipulated
  • Chemically defined media
  • Complex post-translational modifications
  • Stable production strains
  • Durability
  • Ease of scaling up
  • Low protein production cost

At Creative BioMart, our expertise in protein expression extends to both Saccharomyces. cerevisiae and Pichia. pastoris expression systems. Expression of recombinant proteins in S. cerevisiae can be done using three types of vectors:

YIp
  • Yeast integrating plasmid
  • Replicating single copy plasmid
  • For integration into genomic locus
YCp
  • Yeast centromeric plasmid
  • Replicating single copy plasmid
  • For complementation studies
YEp
  • Yeast episomal plasmid
  • Replicating multi-copy plasmid
  • For recombinant protein production, multicopy suppression studies, etc.

Saccharomyces. cerevisiae:

  • Full genomic sequence known
  • Well developed genetic methods
  • About 6000 genes
  • A model to other eukaryotes
  • GRAS
  • No toxins
  • Ability to secrete proteins
  • Capacity for post-translational modifications

Besides, methylotrophic Pichia.pastoris is a distinguished system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per liter of culture. If you're looking for protein production via fed-batch pichia processes, we have the experienced and credentialed staff to move your project forward.

Creative BioMart’s custom production service offers choices of constitutive and inducible expressions with process optimization and scale up development, suitable for research projects at any stage or industry manufacture scale.

 
Step Service Description Timeline Deliverables
1 Gene synthesis plus codon optimization 1-2 weeks
  • Purified protein [supplied in liquid or lyophilized form]
  • Gene sequencing report
  • QC report
  • SDS-PAGE/ HPLC/ SEC
  • Activity data if applicable
2 Clone target gene into expression vector 1 week
3 Transforming Pichia pastoris, Saccharomyces cerevisiae 1week
4 Protein expression and purification 1-2 weeks
5 Optional services [secondary purification, tag removal, endotoxin removal, protein labeling, activity assay, protein analysis, etc.] 1-2 weeks
6 Quality Control <1 week
 

Feel free to send us description of your target or desired outcome of the project, our scientist will guide you through the project from design to delivery and provide expert consulting at no additional charge.

Welcome to contact us or send an email to info@creative-biomart.com for project quotations and more detailed information.

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