Active Recombinant COVID-19 Spike S1 protein (HV69-70del), His-tagged
Cat.No. : | Spike-4885V |
Product Overview : | Recombinant COVID-19 protein (HV69-70del)(Val 16 - Arg 685), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized SARS-CoV-2 S1 protein (HV69-70del), His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.1-2 ng/mL.Immobilized SARS-CoV-2 S1 protein (HV69-70del), His Tag at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL.Immobilized SARS-CoV-2 S1 protein (HV69-70del), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag with a linear range of 0.4-13 ng/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 76.7 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Val 16 - Arg 685 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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