Active Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated
Cat.No. : | FCGR2B-324H |
Product Overview : | Biotinylated Recombinant Human FCGR2B Protein(Ala 46-Ile 224) (NP_001002274.1) was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. |
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Source : | HEK293 |
Species : | Human |
Tag : | His&Avi |
Form : | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its ability to bind human IgG1 in functional ELISA. 2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated bound to Avastin was 0.02 μM. |
Molecular Mass : | The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation. |
Protein length : | Ala 46-Ile 224 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | FCGR2B Fc fragment of IgG, low affinity IIb, receptor (CD32) [ Homo sapiens ] |
Official Symbol : | FCGR2B |
Synonyms : | FCGR2B; CD32; CD32B; CDw32; fcRII-b; Fc gamma RIIb; FCG2; FCGR2; IGFR2; |
Gene ID : | 2213 |
mRNA Refseq : | NM_001002273.2 |
Protein Refseq : | NP_001002273.1 |
MIM : | 604590 |
UniProt ID : | P31994 |
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◆ Lysates | ||
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FCGR2B-1942RCL | Recombinant Rat FCGR2B cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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