Active Recombinant Human NRG1 Protein, His-tagged

• Cat.No.: NRG1-01H
• Product Overview: Recombinant human NRG1 (20-241aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatographytechniques.

Specification

• Description: The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD).
• Source: Insects cells
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 15 ng/mL.
• Molecular Mass: 25.1kDa(231aa)
• AA Sequence: SGKKPESAAGSQSPALPPRLKEMKS QESAAGSKLVLRCETSSEYSSLRFK WFKNGNELNRKNKPQNIKIQKKPGK SELRINKASLADSGEYMCKVISKLG NDSASANITIVESNEIITGMPASTE GAYVSSESPIRISVSTEGANTSSST STSTTGTSHLVKCAEKEKTFCVNGG ECFMVKDLSNPSRYLCKCPNEFTGD RCQNYVMASFYSTSTPFLSLPE
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Protein length: 20-241

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Homo sapiens (human) ]
• Official Symbol: NRG1
• Synonyms: NRG1; neuregulin 1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2; pro-neuregulin-1, membrane-bound isoform; acetylcholine receptor-inducing activity; glial growth factor 2; heregulin, alpha (45kD, ERBB2 p185-activator); neu differentiation factor; pro-NRG1; sensory and motor neuron derived factor
• Gene ID: 3084
• mRNA Refseq: NM_013958
• Protein Refseq: NP_039252
• MIM: 142445
• UniProt ID: Q02297

Reviews

08/12/2022

Excellent results, very consistent.

01/27/2021

Worked great in assays.

01/02/2019

Great product, very reliable.

Q&As

How is NRG1 implicated in neural development and synaptic plasticity?

NRG1 is essential for neural development, influencing neuronal migration, myelination, and synapse formation. It also plays a role in synaptic plasticity, modulating neurotransmission and synaptic strength.

What role does NRG1 play in cardiac development and function?

In the heart, NRG1 is involved in the regulation of cardiac development, including trabeculation. It also has protective effects on cardiac muscle cells and can influence heart repair mechanisms.

Are there any associations between NRG1 mutations or dysregulation and diseases?

Dysregulation of NRG1 signaling has been implicated in several diseases, including schizophrenia, multiple sclerosis, and certain types of cancers. Genetic variations in the NRG1 gene have been associated with increased risk for some of these conditions.

What are the therapeutic implications of targeting NRG1 in various diseases?

Given the diverse roles of NRG1 in various physiological processes, it presents as a potential therapeutic target. For instance, recombinant NRG1 has been explored for heart failure treatment, and its modulation is being studied in the context of neural disorders and cancers.

Are there different isoforms of NRG1, and how do they differ in function?

Yes, there are multiple isoforms of NRG1, generated through alternative splicing. These isoforms can differ in their tissue distribution, receptor binding, and functional roles.

How does NRG1 interact with its receptor, and what are the downstream signaling pathways activated?

NRG1 primarily interacts with the ErbB family of tyrosine kinase receptors, especially ErbB3 and ErbB4. Upon binding, it activates downstream pathways such as the PI3K/Akt and MAPK pathways.

What is the primary biological function of the NRG1 protein?

NRG1 is a versatile signaling molecule that plays crucial roles in the development and function of multiple organ systems, including the nervous system, heart, and breast.