Active Recombinant Mouse Osm Protein, His-tagged

• Cat.No.: Osm-841M
• Product Overview: Recombinant mature form of mouse OSM (P53347) (Met 1-Arg 206) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Asn 25
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 50-200 ng/mL. 2. Measured in a cell proliferation assay using NIH3T3 cells. The ED50 for this effect is typically 0.8-4 ng/mL
• Molecular Mass: The secreted recombinant mouse OSM comprises 193 amino acids and has a calculated molecular mass of 22 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 35-40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Met1-Arg206

Gene Information

• Gene Name: Osm oncostatin M [ Mus musculus ]
• Official Symbol: Osm
• Synonyms: OSM; oncostatin M; oncostatin-M; OncoM
• Gene ID: 18413
• mRNA Refseq: NM_001013365
• Protein Refseq: NP_001013383
• Pathway: Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathwa
• Function: cytokine activity; cytokine activity; growth factor activity; oncostatin-M receptor binding

Reviews

11/25/2020

Outstanding protein-protein interaction studies, vital for our projects.

03/02/2020

High-quality protein characterization, fundamental for our experiments.

02/27/2019

Expertise in peptide synthesis, accelerates our peptide-based research.

Q&As

What is the impact of OSM on cancer development and progression, particularly in terms of tumor microenvironment modulation?

OSM can influence cancer progression by altering the tumor microenvironment and affecting tumor cell behavior.

How does OSM (Oncostatin M) regulate inflammatory and immune responses in the body?

OSM plays a key role in regulating inflammatory and immune responses, modulating cytokine production and immune cell activity.

How does OSM contribute to wound healing and tissue regeneration processes?

OSM aids in wound healing and tissue regeneration, promoting cell growth and repair mechanisms.

What role does OSM play in the pathogenesis of autoimmune diseases like rheumatoid arthritis?

OSM is implicated in autoimmune diseases, contributing to inflammation and joint damage in conditions like rheumatoid arthritis.

How does OSM influence liver fibrosis and hepatic diseases?

OSM is involved in liver fibrosis, impacting the development and progression of hepatic diseases.

What are the mechanisms by which OSM modulates cellular growth, differentiation, and apoptosis?

OSM regulates cellular processes like growth, differentiation, and apoptosis, affecting various tissue functions.

How do alterations in OSM expression or signaling affect cardiovascular health and disease?

Changes in OSM signaling can impact cardiovascular health, influencing disease development and progression.