Active Recombinant Rat Epha4 protein, hFc-tagged
Cat.No. : | Epha4-7439R |
Product Overview : | Recombinant Rat Epha4 protein(D3ZZK3)(Met1-Tyr547), fused with hFc tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Rat |
Tag : | Fc |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its binding ability in a functional ELISA. Immobilized mouse EFNA5-His at 10 μg/ml (100 μl/well) can bind Rat EPHA4-Fc, The EC50 of Rat EPHA4-Fc is 19.8-46.3 ng/ml. |
Molecular Mass : | The recombinant rat EPHA4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 769 amino acids and has a predicted molecular mass of 85.3 kDa. The apparent molecular mass of the protein is approximately 98 kDa in SDS-PAGE under reducing conditions. |
Protein length : | Met1-Tyr547 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 98 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Epha4 Eph receptor A4 [ Rattus norvegicus ] |
Official Symbol : | Epha4 |
Synonyms : | EPHA4; Eph receptor A4; RGD1560587; |
Gene ID : | 316539 |
mRNA Refseq : | NM_001162411 |
Protein Refseq : | NP_001155883 |
Products Types
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EPHA4-3385H | Active Recombinant Human EPHA4 Protein, GST/His-tagged | +Inquiry |
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◆ Lysates | ||
EPHA4-1192CCL | Recombinant Cynomolgus EPHA4 cell lysate | +Inquiry |
EPHA4-2136MCL | Recombinant Mouse EPHA4 cell lysate | +Inquiry |
EPHA4-1933HCL | Recombinant Human EPHA4 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewThe results interpretation process with this protein reagent is straightforward, making it suitable for beginners as well.
The composition ratio of the reagent is highly accurate, ensuring the reliability of the experimental results.
Astounding in both reliability and steadfastness, it confers irrefutable persuasion to the experiment.
Q&As (7)
Ask a questionEPHA4 is a member of the ephrin receptor family of protein kinases that plays an important role in neural development and synaptic plasticity. It is involved in axon guidance, neuronal migration, and cell proliferation.
EPHA4 plays a critical role in neuronal development as a member of the Eph receptor family, which are receptor tyrosine kinases involved in various cellular processes. Specifically, EPHA4 regulates axon growth and guidance, acting as a repulsive cue to guide axons in the developing nervous system. Its involvement extends to synaptic plasticity and neuronal survival. Dysregulation of EPHA4 has been linked to neurological disorders, including Alzheimer's disease and neuropathic pain. Further research on the precise mechanisms and downstream signaling pathways of EPHA4 will deepen our understanding of its role in neuronal development and potential therapeutic applications.
EPHA4 expression is tightly regulated during nervous system development, with its expression gradually increasing during embryonic development. Additionally, EPHA4 expression is spatially regulated, with high levels in specific regions of the nervous system. Regulation of EPHA4 expression is mediated by several mechanisms, including transcriptional and post-transcriptional mechanisms.
EPHA4 is essential for normal synaptic plasticity, with its activation leading to the modulation of various synaptic proteins, such as AMPA receptors, NMDA receptors, and EphB receptors. Dysregulation of EPHA4-mediated signaling has been implicated in multiple neurodegenerative disorders, affecting both synaptic plasticity and axon growth.
EPHA4 signaling is initiated by binding to its ligands, which leads to activation of downstream signaling pathways. Several signaling pathways are activated by EPHA4, including the MAP kinase pathway, the PI3K-Akt pathway, and the RhoA-ROCK pathway, which play critical roles in the regulation of actin cytoskeleton dynamics.
EPHA4 has emerged as a promising therapeutic target for various neurodegenerative diseases, and several pharmacological inhibitors have been developed to target EPHA4 signaling. These inhibitors have shown efficacy in preclinical models of several neurodegenerative diseases, suggesting their potential in clinical applications.
There is growing evidence that genetic variants in the EPHA4 gene may contribute to the susceptibility to neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. These variants have been shown to modify EPHA4 expression and function, and may contribute to disease pathogenesis via several mechanisms, including synaptic dysfunction and axon degeneration.
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