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Active Recombinant Mouse Kit Ligand

Cat.No. : Kitl-307M
Product Overview : Recombinant mouse Stem Cell Factor (Kitl) is involved in survival, differentiation and activation of myeloid, erythroid, lymphoid, mast cells, eosinophils and other progenitor cells. mKitl signals through its receptor, tyrosine kinase Kitl R/c-kit. It is produced in E.Coli as a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18,309 Daltons.
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Cat. No. : Kitl-307M
Description : Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). Kitl can exist both as a transmembrane protein and a soluble protein.
Species : Mouse
Source : E.coli
Form : Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM Sodium phosphate buffer pH=6.5.
Purity : Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : Recombinant Mouse Kitl is fully biologically active when compared to standard. The ED50, as determined by the dose-dependent stimulation of Human TF-1 cells, is 5.5 ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 98% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : KEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Melanogenesis; Pathways in cancer
Gene Name : Kitl kit ligand [ Mus musculus ]
Official Symbol : Kitl
Synonyms : Kitl; kit ligand; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; Kitlg; Steel; contrasted
Gene ID : 17311
mRNA Refseq : NM_013598
Protein Refseq : NP_038626
UniProt ID : P20826
Chromosome Location : 10 D1; 10 57.0 cM
Function : Growth factor activity; Identical protein binding; Stem cell factor receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does KITLG influence stem cell factor (SCF) signaling pathways? 11/10/2022

Overexpression or mutations in KITLG and its receptor have been implicated in various cancers, including gastrointestinal stromal tumors (GISTs), mastocytosis, and certain leukemias.

What are the implications of KITLG in oncogenesis and tumor progression? 07/20/2022

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

What role does KITLG play in melanogenesis and pigmentation? 01/17/2022

Dysregulation or mutations in KITLG have been associated with certain disorders like piebaldism, a genetic condition characterized by unpigmented patches of skin and hair.

Are there any known disorders associated with mutations or dysregulation of KITLG? 06/22/2020

Upon binding to c-KIT, KITLG activates the SCF signaling pathways, which include the MAPK, PI3K, and JAK/STAT pathways, influencing cell survival, proliferation, and differentiation.

What is the primary biological function of KITLG in the hematopoietic system? 04/12/2020

KITLG binds to the c-KIT receptor, a tyrosine kinase receptor, leading to its dimerization and autophosphorylation, which activates downstream signaling pathways.

How has KITLG been explored as a therapeutic target or agent in clinical settings? 02/10/2020

Given its role in cell proliferation and differentiation, KITLG has been explored as a therapeutic agent in stem cell transplantation settings, and its pathway is a target in certain cancer treatments.

How does KITLG interact with its receptor, c-KIT? 12/21/2019

KITLG and its receptor c-KIT are involved in melanogenesis. Their interaction regulates the proliferation and migration of melanocytes, influencing skin, hair, and eye pigmentation.

Customer Reviews (3)

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Reviews
01/23/2022

    Reliable for immunoprecipitation assay.

    03/01/2021

      No contamination, very clean.

      02/09/2020

        Worked perfectly in Western.

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