| Description : |
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Source : |
E. coli |
| Species : |
Mouse |
| Bio-activity : |
The ED50 is 15 ng/mL, corresponding to a specific activity of 6.7 × 10^4 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation. |
| Molecular Mass : |
18.3 kDa |
| Protein length : |
Lys26-Ala189 |
| Endotoxin : |
Less than 0.01 ng/μg protein as determined by the LAL method |
| Purity : |
> 98%, as determined by Coomassie stained SDS-PAGE. |
| Stability : |
Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
Protein formulated and vialed at >50 μg/mL in specified buffer |
| Storage Buffer : |
10 mM NaHPO4 pH 7.2, 0.15 M NaCl |
