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Home > Products > Recombinant Protein > Others > Recombinant Aequorea victoria GFP Protein, His-tagged

Recombinant Aequorea victoria GFP Protein, His-tagged


Product Description

Cat.No.

GFP-03A

Product Name

Recombinant Aequorea victoria GFP Protein, His-tagged

Product Overview

A DNA sequence encoding the Aequorea victoria GFP (Ser 2-Lys 238) was expressed, with a polyhistide tag at the N-terminus.

Description

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.

Source

E. coli

Species

Aequorea victoria

Tag

His

Predicted N Terminal

Met

Form

Lyophilized from sterile PBS, pH 7.5

Molecular Mass

The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7KDa. It migrates as an 35 kDa band in SDS-PAGE under reducing conditions as predicted.

Purity

> 90 % as determined by SDS-PAGE

Storage

Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products.




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  • 45-16 Ramsey Road, Shirley, NY 11967, USA
    USA: 1-631-559-9269
    Europe: 44-207-048-3343
    FAX: 1-631-938-8127
    EMAIL: info@creative-biomart.com

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