Recombinant Aequorea victoria GFP (AAB65663) (Ser 2-Lys 238), fused with a polyhistide tag at the N-terminus, was produced in E. coli.
Lyophilized from 0.2μm filtered solution of PBS, pH 7.5
The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an 35 kDa band in SDS-PAGE under reducing conditions as predicted.
< 1.0 eu per μg of the protein as determined by the LAL method.
Samples are stable for up to twelve months from date of receipt at -70oC.
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.