Recombinant Human CREB produced inE.Coliis a non-glycosylated, polypeptide chain containing amino acids and 2-328 having a molecular mass of 81 KD. This protein is the full-length form and is produced as a maltose binding protein (MBP) fusion protein with a poly His-tag.CREB is purified by proprietary chromatographic techniques.
CREB (cAMP response element-binding) proteins are transcription factors which bind to certain sequences called cAMP response elements (CRE) in DNA and thereby increase or decrease the transcription of certain genes. CREB is highly related (in structure and function) to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins. CREB has many functions in many different organs although most of its functions have been studied in relation to the brain. CREB is also thought to be involved in the growth of some types of cancer. In humans.
Lyophilized freeze dry powder.
Greater than 95% as determined by SDS-PAGE.
CREB is supplied as lyophilized freeze dry powder without additives.
CREB is phosphorylatable in vitro, using either recombinant Protein Kinase A, or Rsk immunoprecipitated from stimulated cells. This phosphorylation can be monitored by ELISA using CREB [pS133] phosphoELISA or by Western blotting using an anti-CREB [pS133] phosphorylation site specific antibody in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.