Active Recombinant Bovine Interferon, Gamma

• Cat.No.: IFNG-61B
• Product Overview: Recombinant Bovine IFN-γ was expressed inYeast.MW= 16.9 kDa(143 aa).

Specification

• Cat. No.: IFNG-61B
• Description: Interferon-gamma (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities.
• Source: Yeast.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: QGQFFREIEN LKEYFNASSP DVAKGGPLFS EILKNWKDES DKKIIQSQIV SFYFKLFENL KDNQVIQRSM DIIKQDMFQK FLNGSSEKLE DFKKLIQIPV DDLQIQRKAI NELIKVMNDL SPKSNLRKRK RSQNLFRGRR AST.
• Bioactivity: The biological activity was measured in a cell activation assay using the Griess reaction as an indicator of nitric oxide production by bovine PBMC. The ED50 for this effect is typically 50 - 60 ug/ml.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IFNG interferon, gamma [ Bos taurus ]
• Synonyms: interferon, gamma; IFNG; boIFNG; IFN-gamma; interferon-gamma; interferon gamma type 2
• Gene ID: 281237
• mRNA Refseq: NM_174086.1
• Protein Refseq: NP_776511.1
• UniProt ID: P07353
• Chromosome Location: 5q22-q24
• Pathway: Allograft rejection; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Proteasome; Regulation of autophagy; Systemic lupus erythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway; Type I diabetes mellitus
• Function: cytokine activity; interferon-gamma receptor binding